Development of an analytical method for individual boron concentration determination from combination of BSH and BPA in human plasma for BNCT

• Project/Area Number: 25350586
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical engineering assessment
• Research Institution: Japan Chemical Analysis Center
• Principal Investigator: BI CHUNLEI 公益財団法人日本分析センター, その他部局等, 研究員 (80647321)
• Co-Investigator(Kenkyū-buntansha): YAMAGUCHI YURIE 日本分析センター, その他部局等, 研究員 (30639977) ; BAMBA SHIGERU 日本分析センター, その他部局等, 研究員 (40544432) ; OSIMA MASUMI 日本分析センター, その他部局等, 研究員 (40354815) ; MORIMOTO TAKAO 日本分析センター, その他部局等, 研究員 (40544359) ; KUMADA HIROAKI 筑波大学, 医学医療系, 准教授 (30354913)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: BNCT / BSHとBPA併用 / LC/MS / 定量分析 / ICP-MS / ホウ素濃度 / 併用投与 / ホウ素薬剤 / ホウ素同位体濃度 / 定量分析法 / BSH / BPA / 分離条件 / ホウ素同位体

Outline of Final Research Achievements

For improvement of the therapeutic efficacy, combinated medication of both sodium borocaptate (BSH) and p-boronophenylalanine (BPA) enriched with 10B, are applied in BNCT clinical studies at present, because this technology loaded of BSH and BPA simutaneously is expected to increase the accumulation of boron concentration in the tumors. Therefore, it is required to measure the individual boron concentration derived from the two different compounds respectively for the accurate dose estimation by neutron irradiation. In this study, an analytical method on the separation of the two boron compounds and individual concentration determination by using a liquid chromatography mass spectrometry (LC/MS) has been developed and the applicability to human plasma samples has been also studied. This analytical method is more practical and easier for individual born concentration determination from combination of BSH and BPA for BNCT. Furthermore, animal injection experiment is under research.

Research Products

• [Journal Article] Study of Charged Particle Activation Analysis (I): Determination Sensitivity for Single Element Samples (2016)
  • Author(s): M. Oshima, Y. Yamaguchi, W. Muramatsu, H. Amano, C. Bi, H. Seto, S. Bamba, and T. Morimoto
  • Journal Title: Journal of Radioanalytical and Nuclear Chemistry Volume: 308 Issue: 2 Pages: 711-719
  • DOI: 10.1007/s10967-015-4505-7
  • Peer Reviewed / Open Access
• [Presentation] Separation and quantitation of BSH and BPA applied in human plasma by liquid chromatography-mass spectrometry (2015)
  • Author(s): 畢春蕾
  • Organizer: Ⅷ Young Researchers BNCT Meeting
  • Place of Presentation: Pavia, Italy
  • Year and Date: 2015-09-13 – 2015-09-17
• [Presentation] Separation and quantitation of BSH and BPA applied in human plasma by liquid chromatography-mass spectrometry (2015)
  • Author(s): 畢春蕾
  • Organizer: 8th Young Researchers BNCT Meeting
  • Place of Presentation: Pavia, Italy
  • Year and Date: 2015-09-13
  • Int'l Joint Research
• [Presentation] Development of automatic analysis device for determination of boron in blood samples (2015)
  • Author(s): 山口友理恵
  • Organizer: 8th Young Researchers BNCT Meeting
  • Place of Presentation: Pavia, Italy
  • Year and Date: 2015-09-13
  • Int'l Joint Research
• [Presentation] A method for individual quantitation of the combined boronophenylalanine and borocaptate by liquid chromatography-electrospray ionization-mass spectrometry (2014)
  • Author(s): 畢春蕾
  • Organizer: 16th International Congress on Neutron Capture Therapy
  • Place of Presentation: Helsinki, Finland