Effect of low-intensity pulsed ultrasound on skeletal muscle regeneration after damage in a mouse model
| Project/Area Number |
25350620
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Kitasato University |
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Principal Investigator |
SAKAMOTO Miki 北里大学, 医療衛生学部, 講師 (40365177)
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| Co-Investigator(Kenkyū-buntansha) |
TADANO Chigaya 東邦大学, 医学部, 講師 (40261094)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 筋損傷 / 低出力パルス超音波療法 / 筋再生 / リハビリテーション / 物理療法 / 骨格筋再生 / 低出力超音波療法 |
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| Outline of Final Research Achievements |
The aim of this study was to examine the effects of Low-intensity pulsed ultrasound (LIPUS) on the regenerative process of damaged muscle in a mouse model. Female ICR mice (12-week-old) were divided into the control group, muscle injury group (LIPUS-) and LIPUS exposure after muscle injury (IPUS+) group. To induce muscle injury, cardiotoxin was injected into the left tibialis anteriol(TA) muscle. TA muscles were excised at 3, 5, 7, 14, and 21 days after injury. In the results, there was no significant difference in the mean fiber cross-sectional area (CSA) between the LIPUS- group and the LIPUS+ group. However, the number of larger myofibers tended to increase in the LIPUS+ group compared with the LIPUS- group at 7, 14, and 21 days post-injury. The expression of p-p70S6K and the phosphorylation ratio of p70S6K were significantly higher in the LIPUS+ group compared with the LIPUS- group at 5 days post-injury. These results suggest that LIPUS promotes skeletal muscle regeneration.
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Report
(4 results)
Research Products
(9 results)
