Molecular aspects of skeletal muscle plasticity in response to extracellular stimuli -Therapeutic applications for locomotive syndrome-

• Project/Area Number: 25350641
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Rehabilitation science/Welfare engineering
• Research Institution: Toyohashi Sozo University
• Principal Investigator: OHNO Yoshitaka 豊橋創造大学, 保健医療学部, 講師 (80440808)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 細胞・組織 / ストレス / 筋肥大 / 骨格筋

Outline of Final Research Achievements

The purpose of this study was to investigate the effects of extracellular stimuli, such as mechanical loading and heat stress, on the plasticity of skeletal muscle. Absence of heat shock transcription factor 1 (HSF1) gene partially attenuated the overloading-associated skeletal muscle hypertrophy in mice. Heat stress-associated increase in muscle mass and muscle satellite cells was also suppressed by HSF1-deficiency. Furthermore, HSF1-deficiency depressed heat stress-associated activation of Akt/p70 S6 kinase pathway. In addition, upregulation of heat shock proteins (HSPs) in skeletal muscle was attenuated by the absence of HSF1. These observations suggested that HSF1-mediated stress response, including the upregulation of HSPs, may play a key role in skeletal muscle hypertrophy induced by mechanical overloading and heat stress.

Research Products

• [Journal Article] Deficiency of heat shock transcription factor 1 suppresses heat stress-associated increase in slow soleus muscle mass of mice (2015)
  • Author(s): Ohno Y, Egawa T, Yokoyama S, Nakai A, Sugiura T, Ohira Y, Yoshioka T, Goto K
  • Journal Title: Acta Physiol (Oxf) Volume: 215 Issue: 4 Pages: 191-203
  • DOI: 10.1111/apha.12600
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Involvement of AMPK in regulating slow-twitch muscle atrophy during hindlimb unloading in mice. (2015)
  • Author(s): Egawa T, Goto A, Ohno Y, Yokoyama S, Ikuta A, Suzuki M, Sugiura T, Ohira Y, Yoshioka T, Hayashi T, Goto K.
  • Journal Title: Am J Physiol Endocrinol Metab. Volume: 309 Issue: 7 Pages: E651-E662
  • DOI: 10.1152/ajpendo.00165.2015
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Loading-associated expression of TRIM72 and caveolin-3 in antigravitational solues muscle in mice (2014)
  • Author(s): Ohno Y, Sugiura T, Ohira Y, Yoshioka T, Goto K
  • Journal Title: Physiological Reports Volume: 2 Issue: 12 Pages: e12259-e12259
  • DOI: 10.14814/phy2.12259
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 温熱刺激による骨格筋肥大と生理学的意義 (2014)
  • Author(s): 大野善隆
  • Journal Title: 物理療法科学 Volume: 21 Pages: 14-16
  • Acknowledgement Compliant
• [Journal Article] AICAR-induced activation of AMPK negatively regulates myotube hypertrophy through HSP72-mediated pathway in C2C12 skeletal muscle cells (2014)
  • Author(s): Egawa T. Ohno Y, Goto A, Ikuta A, Suzuki M, Ohira T, Yokoyama S, Sugiura T, Ohira Y, Yoshioka T, Goto K
  • Journal Title: Am J Physiol Endo Metabo Volume: 306 Issue: 3 Pages: E344-E354
  • DOI: 10.1152/ajpendo.00495.2013
  • Peer Reviewed / Open Access
• [Journal Article] Microcurrent electrical nerve stimulation facilitates regrowth of mouse soleus muscle (2013)
  • Author(s): Ohno, Y., Fujiya, H., Goto, A., Nakamura, A., Nishiura, Y., Sugiura, T., Ohira, Y., Yoshioka, T., Goto, K
  • Journal Title: Intl. J. Med. Sci Volume: Vol.10, No.10 Issue: 10 Pages: 1286-1294
  • DOI: 10.7150/ijms.5985
  • NAID: 120005350264
  • Peer Reviewed
• [Journal Article] Regeneration of injured skeletal muscle in heat shock transcriptional factor 1-null mice (2013)
  • Author(s): Nishizawa S, Koya T, Ohno Y ; Goto A, Ikuita A, Suzuki M, Ohira T, Egawa T, Nakai A, Sugiura T, Ohira Y, Yoshioka T, Bepp u M, Goto K
  • Journal Title: Physiol Rep Volume: 1 Issue: 3
  • DOI: 10.1002/phy2.71
  • Peer Reviewed
• [Journal Article] Heat shock transcriptional factor 1-deficiency attenuates overloading-associated hypertrophy of mouse soleus muscle (2013)
  • Author(s): Koya T, Nishizawa S, Ohno Y, Goto A, Ikuita A, Suzuki M, Ohira T, Egawa T, Nakai A, Sugiura T, Ohira Y, Yoshioka T, Bepp u M, Goto K
  • Journal Title: PLoS ONE Volume: 8 Issue: 10 Pages: e77788-e77788
  • DOI: 10.1371/journal.pone.0077788
  • Peer Reviewed
• [Presentation] 筋衛星細胞の増殖が不活動を伴う筋損傷回復遅延に及ぼす影響 (2015)
  • Author(s): 大野善隆，後藤勝正
  • Organizer: 第2回日本基礎理学療法学会学術集会・日本基礎理学療法学会第20回学術大会合同学会
  • Place of Presentation: 神奈川県立保健福祉大学（神奈川県・横須賀市）
  • Year and Date: 2015-11-14
• [Presentation] 温熱刺激による骨格筋増量とHSP110の応答 (2015)
  • Author(s): 大野善隆，後藤勝正
  • Organizer: 第23回日本物理療法学会学術大会
  • Place of Presentation: 神戸学院大学有瀬キャンパス（兵庫県・神戸市）
  • Year and Date: 2015-10-24
• [Presentation] 乳酸が培養骨格筋細胞の分化に及ぼす影響 (2015)
  • Author(s): 大野善隆，杉浦崇夫，大平充宣，吉岡利忠，後藤勝正
  • Organizer: 第70回日本体力医学会大会
  • Place of Presentation: 和歌山県民文化会館，ホテルアバローム紀の国（和歌山県・和歌山市）
  • Year and Date: 2015-09-18
• [Presentation] 熱ショック転写因子1の欠損は温熱刺激による骨格筋肥大を抑制する (2015)
  • Author(s): 大野善隆，後藤勝正
  • Organizer: 第50回日本理学療法学術大会
  • Place of Presentation: 東京国際フォーラム（東京都・千代田区）
  • Year and Date: 2015-06-05
• [Presentation] Suppression of myostatin facilitates the regeneration of injured skeletal muscle in mice under unloading condition (2015)
  • Author(s): Ohno Y., Matsuba Y., Hashimoto N., Sugiura T., Ohira Y., Yoshioka T., Goto K.
  • Organizer: The 11th WORLD CONGRESS of the International Society for ADAPTIVE MEDICINE
  • Place of Presentation: 米子コンベンションセンター（鳥取県・米子市）
  • Year and Date: 2015-05-27
  • Int'l Joint Research
• [Presentation] 骨格筋の量的変化と細胞膜構築タンパク質の発現 (2014)
  • Author(s): 大野善隆，後藤勝正
  • Organizer: 第1回日本基礎理学療法学会学術集会・日本基礎理学療法学会第4回学術大会合同学会
  • Place of Presentation: 名古屋学院大学名古屋キャンパス白鳥学舎（愛知県・名古屋市）
  • Year and Date: 2014-11-15 – 2014-11-16
• [Presentation] 微弱電流刺激による骨格筋量増大におけるタンパク質合成機構に関する基礎的研究 (2014)
  • Author(s): 大野善隆，後藤勝正
  • Organizer: 第22回日本物理療法学会学術大会
  • Place of Presentation: 信州大学医学部附属病院外来棟4階（長野県・松本市）
  • Year and Date: 2014-10-25 – 2014-10-26
• [Presentation] 筋活動量の変化に伴う骨格筋細胞膜タンパク質の応答 (2014)
  • Author(s): 大野善隆，後藤勝正
  • Organizer: 第69回日本体力医学会大会
  • Place of Presentation: 長崎大学文教キャンパス（長崎県・長崎市）
  • Year and Date: 2014-09-19 – 2014-09-21
• [Presentation] 物理的刺激による骨格筋量変化とストレス応答 (2014)
  • Author(s): 大野善隆，後藤勝正
  • Organizer: 第49回日本理学療法学術大会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2014-05-30 – 2014-06-01
• [Presentation] 骨格筋の量的増加に作用する微弱電流刺激とその至適条件 (2013)
  • Author(s): 大野善隆，後藤勝正
  • Organizer: 第48回日本理学療法学術大会
  • Place of Presentation: 名古屋国際会議場（愛知県）
• [Presentation] Mechanical loading-associated changes of membrane protein expression in mouse skeletal muscle (2013)
  • Author(s): Y. Ohno, T. Egawa, A. Ikuta, A. Goto, T. Sugiura, Y. Ohira, T. Yoshioka, K. Goto
  • Organizer: The 34th Annual International Gravitational Physiology Meeting
  • Place of Presentation: 穂の国とよはし芸術劇場プラット（愛知県）
• [Presentation] 温熱刺激による骨格筋肥大と生理学的意義 (2013)
  • Author(s): 大野善隆
  • Organizer: 第21回日本物理療法学会学術大会
  • Place of Presentation: 神奈川県立保健福祉大学（神奈川県）
• [Remarks] 豊橋創造大学保健医療学部理学療法学科生理学研究室ホームページ
  • URL: http://www.sozo.ac.jp/professor/goto_katsumasa/index.html
• [Remarks] 豊橋創造大学保健医療学部理学療法学科生理学研究室ホームページ
  • URL: http://www.sozo.ac.jp/professor/goto_katsumasa/index.html