Novel peptide-dialysis system to assess the effect of physical exercise on the circadian clock
| Project/Area Number |
25350808
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Sports science
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| Research Institution | Hokkaido University |
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Principal Investigator |
Yamanaka Yujiro 北海道大学, 医学(系)研究科(研究院), 助教 (20528343)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | マイクロダイアリシス / マウス / 視交叉上核 / アルギニバゾプレッシン / 行動リズム / アルギニンバゾプレッシン / サーカディアンリズム / 脳ペプチドダイアリシス / 生物時計 / ペプチドダイアリシス / in vivo |
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| Outline of Final Research Achievements |
Brain microdialysis is a powerful technique for measuring a neurotransmitter in extracellular fluid from free moving animals. By using this technique with some modification for measuring a large molecule (neuropeptide), we examined circadian rhythm of neuropeptide released from a central circadian pacemaker of suprachiasmatic nucleus in free moving mice. The mice were placed under a normal light-dark cycle and constant darkness conditions for 2-4 days. During this period, artificial cerebrospinal fluid was perfused at a flow rate of 1 μl/min and the dialysate were collected every 1 h. As a result, circadian rhythm of AVP in dialysate was detected in some mice that the dialysis probe was inserted close to the SCN. The highest value was observed in early subjective day (ZT4). However, in other mice, circadian rhythms of AVP in dialysate were not detected. Technical improvement would be needed in future studies.
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Report
(4 results)
Research Products
(10 results)
