| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Cellular protein synthesis is believed to be antagonistically regulated by mTOR and AMPK signaling pathways. In the present study, we examined the relationship between mTOR/p70S6K and AMPK in response to mechanical stretch. Phosphorylation of p70S6K and AMPK were concomitantly increased by mechanical stretch. Stimulation of the mTOR pathway by adding leucine and insulin increased the phosphorylation of p70S6K without inactivation of AMPK. In contrast, pharmacological inhibition of AMPK by the addition of compound C enhanced the phosphorylation of p70S6K in response to mechanical stretch. Activation of AMPK by the addition AICAR reduced the phosphorylation of p70S6K in response to mechanical stretch. In conclusion, crosstalk between mTOR and AMPK signaling was not tightly regulated in response to physiological stimuli, such as mechanical stress and/or nutrients. However, pharmacological activation or inactivation of AMPKα affected the mTOR/p70S6K signaling pathway.
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