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Basic study on high-altitude training: Molecular mechanisms of neurotransmitters in arterial chemoreceptor

Research Project

Project/Area Number 25350823
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Sports science
Research InstitutionKokushikan University

Principal Investigator

KUSAKABE TATSUMI  国士舘大学, 体育学部, 教授 (80117663)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Yoshio  岩手大学, 農学部, 教授 (10252123)
TAKAHASHI Masahiro  横浜市立大学, 医学部, 講師 (50315800)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords低酸素 / 高血圧 / 化学受容器 / 神経伝達物質 / 細胞内カルシウム / 高地トレーニング / 頚動脈小体 / 免疫組織化学
Outline of Final Research Achievements

The carotid body(CB) of hypertensive rat (SHR) was enlarged in the reconstructed images compared with that of normotensive rat (WKY). Immunoreactivity for tyrosine hydroxylase (TH) and dopamine β-hydroxylase (DBH) in CB was mainly observed in chemoreceptor cells, and the number of chemoreceptor cells with strong DBH immunorectivity was increased in SHR. The present results suggested that the signal transduction from CB is regulated by noradrenaline in chemoreceptor cells under hypertensive conditions. 5-HT did not change intracellular Ca2+ [Ca2+]i in chemoreceptor cells, whereas induced repetitive [Ca2+]i increases during hypoxia. The frequency of hypoxia-induced [Ca2+]i changes was enhanced in the response of 5-HT. These results suggest that 5-HT may increase its own hypoxic chemosensitivity. 5-HT did not change [Ca2+]i in arteriole smooth muscle cells. However, 5-HT induced [Ca2+]i increases in pericytes in capillary.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (13 results)

All 2015 2014 2013

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Acknowledgement Compliant: 5 results,  Open Access: 2 results) Presentation (5 results)

  • [Journal Article] Sympathetic regulation of vascular tone via noradrenaline and serotonin in the rat carotid body as revealed by intracellular calcium imaging2015

    • Author(s)
      Takuya Yokoyama, Nobuaki Nakamuta, Tatsumi Kusakabe, Yoshio Yamamoto
    • Journal Title

      Brain Research

      Volume: 1596 Pages: 126-135

    • DOI

      10.1016/j.brainres.2014.11.037

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Sympathetic and sensory innervations of small intensely fluor escent (SIF) cells in rat superior cervical ganglion2015

    • Author(s)
      Takaki, F., Nakamura, N., Kusakabe, T., Yamamoto,Y
    • Journal Title

      Cell Tissue Res.

      Volume: 359 Issue: 2 Pages: 441-451

    • DOI

      10.1007/s00441-014-2051-1

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Serotonin-mediated modulation of hypoxia-induced intracellular calcium responses in glomus cells isolated from rat carotid body2015

    • Author(s)
      Takuya Yokoyama, Nobuaki Nakamuta, Tatsumi Kusakabe, Yoshio Yamamoto
    • Journal Title

      Neuroscience Letters

      Volume: 597 Pages: 149-153

    • DOI

      10.1016/j.neulet.2015.04.044

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Immunohistochemical localization of dopamine D2 receptor in the rat carotid body2015

    • Author(s)
      J.Wakai, A.Takayama, T.Yokoyama, N.Nakamura, T.Kusakabe, Y.Yamamoto
    • Journal Title

      Acta Histochem.

      Volume: 117 Issue: 8 Pages: 784-789

    • DOI

      10.1016/j.acthis.2015.07.007

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Vesicular glutamate transporter 2-immunoreactive afferent nerve terminals in the carotid body of the rat2014

    • Author(s)
      Yokoyama, T., Nakamura, N., Kusakabe, T., Yamamoto,Y
    • Journal Title

      Cell Tissue Res.

      Volume: 358 Issue: 1 Pages: 271-275

    • DOI

      10.1007/s00441-014-1921-x

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Short-term hypoxia transiently increases dopamineβ-hydroxylase immunoreactivity in glomus cells of the rat carotid body2013

    • Author(s)
      Kato K, Yokoyama T, Yamaguchi-Yamada M, Yamamoto Y
    • Journal Title

      Journal of Histochemistry & Cytochemistry

      Volume: 61 Issue: 1 Pages: 55-62

    • DOI

      10.1369/0022155412464639

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Short-term hypoxia increases phosphorylated tyrosine hydroxylase at Ser31 and Ser40 in rat carotid body2013

    • Author(s)
      Kato K, Yamamoto Y
    • Journal Title

      Respiratory Physiology & Neurobiology

      Volume: 185 Issue: 3 Pages: 543-546

    • DOI

      10.1016/j.resp.2012.11.004

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical localization of tryptophan hydroxylase and serotonin transporter in the carotid body of the rat2013

    • Author(s)
      Yokoyama, T., Yamaguchi-Yamada, M., Yamamoto, Y.
    • Journal Title

      Histochem. Cell Biol

      Volume: 印刷中 Issue: 2 Pages: 147-155

    • DOI

      10.1007/s00418-012-1066-5

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] ウサギ頚動脈小体の形態学的および免疫組織化学的特徴2015

    • Author(s)
      横山拓矢、柴田秀史、中牟田伸明、日下部辰三、山本欣郎
    • Organizer
      第158回日本獣医学会
    • Place of Presentation
      北里大学(青森、十和田)
    • Year and Date
      2015-09-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] ラット頚動脈小体の化学受容活性に対するセロトニンによる調節2015

    • Author(s)
      横山 拓矢、中村 伸明、日下部 辰三、山本 欣郎
    • Organizer
      第120回日本解剖学会学術総会
    • Place of Presentation
      神戸国際会議場(神戸)
    • Year and Date
      2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] セロトニンによるラット頚動脈小体化学授与横細胞における低酸素応答の増強2014

    • Author(s)
      横山 拓矢、中村 伸明、日下部 辰三、山本 欣郎
    • Organizer
      第157回日本獣医学会学術総会
    • Place of Presentation
      北海道大学、高等教育推進機構(札幌)
    • Year and Date
      2014-09-11
    • Related Report
      2014 Research-status Report
  • [Presentation] ラット頚動脈小体におけるドーパミンD1、D2受容体の免疫組織化学的検索2014

    • Author(s)
      高木 杏奈、横山 拓矢、中村 伸明、日下部 辰三、山本 欣郎
    • Organizer
      第157回日本獣医学会学術総会
    • Place of Presentation
      北海道大学、高等教育推進機構(札幌)
    • Year and Date
      2014-09-10
    • Related Report
      2014 Research-status Report
  • [Presentation] セロトニンによるラット頚動脈小体細動脈平滑筋における細胞内Ca2+濃度変化2013

    • Author(s)
      横山拓矢、中牟田信明、日下部辰三、山本欣郎
    • Organizer
      第59回日本解剖学会東北・北海道連合支部学術集会
    • Place of Presentation
      札幌
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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