| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Saliva is a useful sample non-invasively collected from body fluid. Our objective in the present study is to search for saliva biomarkers for the differentiation between physical and mental stress for qoality of life. First, we examined rat saliva marker proteins for mental stress by proteome using a rat mental stress model. The increased proteins by mental stress were subjected to liquid chromatography-mass spectrometry/mass spectrometry. We detected the known enzymes and secretary proteins with MW of 20-70KDa in rat saliva proteins. Secondly, we analyzed the biomarkers for physical stress by proteome after treadmill running loading to rats. After the separation by the above same method, the increased and/or decreased proteins by physical stress were used for LC-MS/MS and a comprehensive proteomic analysis (isobaric Tags for Relative and Absolute Quantitation, iTRAQ). We might find candidate biomarkers for mental and/or physical stress.
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