Elucidation of the signal factors to increase the monocarboxylate transporter expression of skeletal muscle and brain by exercise

• Project/Area Number: 25350831
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Sports science
• Research Institution: Osaka University of Health and Sport Sciences
• Principal Investigator: Hamada Taku 大阪体育大学, 体育学部, 教授 (00466294)
• Co-Investigator(Kenkyū-buntansha): HAYASHI Tatsuya 京都大学, 大学院人間・環境学研究科, 教授 (00314211)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 運動 / 脳 / 骨格筋 / 乳酸代謝 / モノカルボン酸輸送担体 / AMPK / 鉄欠乏 / 乳酸 / シグナル因子

Outline of Final Research Achievements

This study was to verify the effect of monocarboxylate transporter (MCT) expression in the brain as skeletal muscle and its signal factors to increase MCT expression by exercise. A single bout of exercise increased the neuron-specific MCT2 expression level in the brain. Moreover, MCT1 and MCT4 isoforms were increased. Moreover, the signal factors that increase the brain region specific MCT expression level were examined. The results indicated a possibility that brain lactate production and/or brain-derived neurotrophic factor (BDNF) may become a signal factor that increases the MCT2 expression level in brain. These results suggest that MCT2 expression level of the brain is increased by exercise. As a result of this adaptation phenomenon, lactate uptake ability in the neurons is increased, this can be expected that brain function may enhance.

Research Products

• [Journal Article] Acute exercise increases brain region-specific expression of MCT1, MCT2, MCT4, GLUT1, and COX IV proteins (2014)
  • Author(s): Takimoto M, Hamada T
  • Journal Title: Journal of Applied Physiology Volume: 116 Issue: 9 Pages: 1238-1250
  • DOI: 10.1152/japplphysiol.01288.2013
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Possible involvement of AMPK in acute exercise- induced expression of monocarboxylate transporters MCT1 and MCT4 mRNA in fast-twitch skeletal muscle (2013)
  • Author(s): Takimoto T, Takeyama M, Hamada T
  • Journal Title: Metabolism Volume: 62
  • Peer Reviewed
• [Journal Article] Regulation of the acute exercise-induced expression of the monocarboxylate transporters MCT1 and MCT4 in skeletal muscle (2013)
  • Author(s): Hamada T, Takimoto M
  • Journal Title: J. Phys. Fitness Sports Med Volume: 2
• [Presentation] Prolonged exercise upregulates expression of MCT1, MCT2, MCT4 mRNA in the cerebral cortex of rat brain (2013)
  • Author(s): Takimoto T, Takeyama M, Hamada T
  • Organizer: Experimental Biology
  • Place of Presentation: Boston Convention Center
  • Invited