Analysis of coronary artery blood flow in diabetic rat using synchrotron radiation coronary microangiography

• Project/Area Number: 25350879
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied health science
• Research Institution: University of Tsukuba
• Principal Investigator: SAKAMOTO HIROAKI 筑波大学, 医学医療系, 講師 (30611115)
• Co-Investigator(Kenkyū-buntansha): SATO Fujio 筑波大学, 医学医療系, 講師 (20375497) ; TOKUNAGA Chiho 筑波大学, 医学医療系, 講師 (30451701) ; HYODO Kazuyuki 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 准教授 (60201729) ; MATSUSHITA Shonosuke 筑波技術大学, 保健科学部, 教授 (70359579)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 放射光 / 糖尿病 / 冠動脈 / ラット / 放射光微小血管造影法 / 冠動脈造影 / in vivoラット

Outline of Final Research Achievements

The purpose of this research is to elucidate the feature of coronary artery in diabetic patients. First of all, we developed a system of synchrotron radiation coronary microangiography (SRCA) in the in vivo rat. SRCA can visualize vessels as small as 50 um in diameter, thus enabling us to evaluate the coronary artery reaction in the in vivo rat. After that, SRCA could confirm the microvascular constriction of the coronary arteries in the in vivo rat after endothelin administration and detect 25 um caliber change of coronary artery.
And also, SRCA could confirm that sympathetic nerve stimulation by cold exposure induced coronary vasoconstriction in the in vivo diabetic rat. Average dimeter of all measured coronary arteries after cold exposure was increased by 9% in the normal rat and decreased by 10% in the DM rats. This result suggests that the biological response function of coronary artery against external stress is low in the in vivo diabetic rats.

Research Products

• [Journal Article] A new technique of in vivo synchrotron radiation coronary microangiography in the rat. (2015)
  • Author(s): Sakamoto H, Matsushita S, Hyodo K, Tokunaga C, Sato F, Hiramatsu Y
  • Journal Title: Acta Radiologica Volume: 56 Issue: 9 Pages: 1105-1107
  • DOI: 10.1177/0284185114549570
  • Peer Reviewed
• [Presentation] Synchrotron radiation coronary microangiography could confirm induction of coronary vasoconstriction by cold exposure in the diabetic rat. (2015)
  • Author(s): Sakamoto H, Matsushita S, Hyodo K, Tokunaga C, Sato F, Hiramatsu Y
  • Organizer: American Heart Association Scientific Sessions 2015
  • Place of Presentation: オーランド（アメリカ合衆国）
  • Year and Date: 2015-11-07
• [Presentation] Synchrotron radiation coronary microangiography for evaluating changes in the caliber of the in vivo rat coronary artery after endothelin administration (2014)
  • Author(s): 32.Hiroaki Sakamoto, Shonosuke Matsushita, Kazuyuki Hyodo, Chiho Tokunaga, Fujio Sato, Yuji Hiramatsu
  • Organizer: American Heart Association Scientific Sessions 2014
  • Place of Presentation: シカゴ（アメリカ合衆国）
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] A newly developed technique of synchrotron radiation coronary micro-angiography in an in-vivo rat (2013)
  • Author(s): Hiroaki Sakamoto, Shonosuke Matsushita, Kazuyuki Hyodo, Chiho Tokunaga, Fujio Sato, Misao Kubota, Kenkichi Tanioka, Yuji Hiramatsu, Yuzuru Sakakibara
  • Organizer: American Heart Association Scientific Sessions 2013
  • Place of Presentation: Dallas Convention Center（テキサス州、米国）
• [Presentation] In vivoラットにおける放射光微小血管造影法を用いた超精細な冠動脈造影 (2013)
  • Author(s): 坂本裕昭、松下昌之助、徳永千穂、佐藤藤夫、平松祐司、榊原 謙
  • Organizer: 第66回日本胸部外科学会定期学術集会
  • Place of Presentation: 仙台国際センター（宮城県）