Effects of hyperbaric hyperoxia on bone formation and fracture healing.

Research Project

Project/Area Number	25350881
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Applied health science
Research Institution	Teikyo University (2014-2015) National Center for Geriatrics and Gerontology (2013)
Principal Investigator	Kawada Shigeo 帝京大学, 医療技術学部, 講師 (20376601)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords	骨折治癒 / 高気圧高酸素 / 骨の石灰化 / 高気圧 / 高酸素 / 石灰化 / 組織酸素分圧 / 骨
Outline of Final Research Achievements	The purpose of this study was to investigate whether hyperbaric hyperoxia(HBO) accelerate fracture healing in mice. In this study, we developed an open femur fracture model in mice. In HBO-exposed mice, radiographic, histological and biomechanical findings revealed that HBO conditions accelerate fracture healing. Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG), a biomarker of oxidative DNA damage, and plasma calcium concentrations did not change due to the HBO conditions. These results suggest that exposure to HBO enhances bone anabolism and accelerates fracture healing without causing oxidative DNA damage or disruption of plasma calcium homeostasis.