Genome-wide analysis of epigenetic events induced by exercise prescription, and the significance in terms of preventive medicine

• Project/Area Number: 25350884
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied health science
• Research Institution: Shinshu University
• Principal Investigator: HASHIMOTO Shigenari 信州大学, 学術研究院医学系, 助教 (90359729)
• Co-Investigator(Kenkyū-buntansha): TANIGUCHI Shunichiro 信州大学, 学術研究院医学系, 教授 (60117166) ; NAKAJIMA Koki 松本大学, 人間健康学部, 教授 (80258954)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 健康増進 / 運動処方 / インターバル速歩 / エピジェネティクス / 炎症 / NFkB遺伝子 / メチル化 / 運動介入実験 / エピジェネティックス / 抗炎症効果 / NFkB / 健康処方 / 遺伝子のメチル化 / 抗炎症

Outline of Final Research Achievements

To improve the health condition of elderly people, Nose et al. have developed an exercise program termed high-intensity interval walking training (IWT). Conventional physical and biochemical markers　have shown the health-promoting effects of IWT. On one hand, we have been also investigating the effects of IWT from molecular-biological points of view.
In this study, we did epigenetical investigation on the effects of IWT with genome-wide DNA methylation assays. Our aim was to uncover genes that respond to IWT through examining DNA methylation changes in samples of IWT subjects. The obtained results indicated that IWT led to epigenetically suppress a pro-inflammatory status, consistently with our previous study on pro-inflammatory ASC gene methylation. We found significant methylation changes in several genes relating to inflammation. In particular, it was note-worthy that NFkB2, one of master genes for inflammation, was hyper-methylated.

Research Products

• [Journal Article] NFkB2 gene as anoble candidate epidemiologically responding to interval walking training. (2015)
  • Author(s): Zhang Y, Hashimoto S, Fujii C, Hida S, Ito K, Matsumura T, Sakaizawa T, Morikawa M, Masuki S, Nose H, Higuchi K, Nakajima K, Taniguchi S
  • Journal Title: Int. J. Sports. Med. Volume: in press Issue: 09 Pages: 769-775
  • DOI: 10.1055/s-0035-1547221
  • Peer Reviewed / Open Access