Role of diet and lifestyles in regulating nonsense mRNA expression and its associated diseases

• Project/Area Number: 25350887
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied health science
• Research Institution: The University of Tokushima
• Principal Investigator: HARADA Nagakatsu 徳島大学, 大学院医歯薬学研究部, 講師 (40359914)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ライフスタイル / ナンセンス型mRNA / 規格外遺伝子 / アウトレット / 予防医学 / ストレス / 高脂肪食 / 運動負荷 / mRNA品質管理 / 食事制限 / 絶食 / AMPK / SCD1 / ルシフェラーゼ / 小胞体ストレス / ナンセンスコドン依存性mRNA分解 / レポーター遺伝子 / 細胞ストレス / NMD / 飽和脂肪酸 / 運動 / 骨格筋 / PTC

Outline of Final Research Achievements

Effect of diet and exercise training on nonsense-mediated mRNA decay (NMD) and nonsense mRNA expression was studied in mice. The results show that high cacao butter diet, but not other high fat diet or exercise training, increased nonsense mRNA expression levels in mouse liver. We revealed that the cacao butter-induced increase in nonsense mRNA levels was not dependent on phosphorylation levels of eIF2alpha and expression levels of NMD factor UPF1 and lipogenic protein SCD1.

Research Products

• [Journal Article] C-terminal region of GADD34 regulates eIF2α dephosphorylation and cell proliferation in CHO-K1 cells. (2016)
  • Author(s): Otsuka R, Harada N, Aoki S, Shirai K, Nishitsuji K, Nozaki A, Hatakeyama A, Shono M, Mizusawa N, Yoshimoto K, Nakaya Y, Kitahata H, Sakaue H
  • Journal Title: Cell stress and chaperones Volume: 21 Issue: 1 Pages: 2940-2940
  • DOI: 10.1007/s12192-015-0633-9
  • Peer Reviewed
• [Presentation] Nonsense-mediated mRNA decay regulates expression of a constitutively active form of sterol regulatory element-binding protein 1. (2015)
  • Author(s): Nagakatsu Harada, Hitomi Taniwaki, Haruka Yonemoto, Takashi Oyamada, Yukie Nara, Haruka Omachi, Ayane Nozaki, Masaki Yoshida, Rie Tsutsumi, Hironori Yamamoto, Hiroshi Sakaue, Yutaka Nakaya
  • Organizer: The 8th Asia-Oceania Conference on Obesity
  • Place of Presentation: Nagoya Congress Center, Nagoya City, Aichi, Japan
  • Year and Date: 2015-10-03
  • Int'l Joint Research