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Search for in vivo substrates and inhibitors of calpain related to Alzheimer's disease

Research Project

Project/Area Number 25350917
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied health science
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

TSUBUKI SATOSHI  国立研究開発法人理化学研究所, 脳科学総合研究センター, 専門職研究員 (20217368)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsアルツハイマー病 / カルパイン / カルパスタチン / 二次元電気泳動 / タンパク質同定 / 質量分析 / 遺伝子改変マウス / 初代培養細胞 / 等電点電気泳動
Outline of Final Research Achievements

I tried to identify in vivo substrates of calpain related to Alzheimer’s disease using hippocampal primary neurons of calpastatin-ko and wild-type mice. I analysed four fractions of homogenates of primary neurons by two dimensional fluorescence difference gel electrophoresis. Also I applied them to the calpain-binding column and identified the adsorbed protein as a in vivo substrate by liquid chromatography-mass spectrometry. And I tried kinetic analysis using the specific antibodies.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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