| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Of secondary structures observed in nucleic acids, guanine quadruplex (G-quadruplex) structures themselves can serve as a functional element in the regulation of gene expression. G-quadruplex consists of stacking of planar guanine tetrads, in which four guanine bases make Hoogsteen hydrogen bonds with incorporating metal ion, such as potassium and sodium, inside the tetrads. Deeper understanding of biological relevant G-quadruplex structure would be helpful for artificial gene regulation by manipulating DNA/RNA structures. Here we demonstrate that guanine-tethered antisense oligonucleotide could inhibit protein synthesis by a sequence specific introduction of hetero DNA-RNA G-quadruplex structures.
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