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Creation of xenonucleic acid switch molecules

Research Project

Project/Area Number 25350962
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biomolecular chemistry
Research InstitutionGunma University

Principal Investigator

Kuwahara Masayasu  群馬大学, 大学院理工学府, 准教授 (40334130)

Co-Investigator(Renkei-kenkyūsha) OBIKA Satoshi  大阪大学, 大学院薬学研究科, 教授 (80243252)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsキセノ核酸 / 核酸アプタマー / スイッチ分子 / 刺激応答性 / 環境応答性
Outline of Final Research Achievements

Switch molecules based on nucleic acid aptamers which can vary their binding activities depending on external stimuli and surroundings are expected to be applicable to drug delivery systems and biosensors. In this study, we attempted to create xenonucleic acid switch molecules, which are triggered by chemical conversions of bridged nucleotides owing to external stimuli and surroundings. Consequently, we found that replacements of bridged nucleotides bearing cytosine base with natural 2'-deoxycytidine at 9th and 15th positions could inactivate the avidity of the aptamer, whereas those with 2'-deoxy-5-methylcytidine retained the binding activity, indicating that the obtained switch molecule will potentially be applicable to detection systems for cytosine methylations.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (42 results)

All 2015 2014 2013

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Acknowledgement Compliant: 2 results) Presentation (33 results) (of which Int'l Joint Research: 7 results,  Invited: 7 results) Book (1 results)

  • [Journal Article] Modified nucleic acid aptamer selections using capillary electrophoresis2015

    • Author(s)
      桑原 正靖、萩原 健太、入澤 祐太、笠原 勇矢
    • Journal Title

      Electrophoresis Letters

      Volume: 59 Issue: 2 Pages: 88-90

    • DOI

      10.2198/electroph.59.88

    • NAID

      130005103929

    • ISSN
      2189-2628, 2189-2636
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro selection of DNA-based aptamers that exhibit RNA-like conformations using a chimeric oligonucleotide library that contains two different xeno-nucleic acids.2015

    • Author(s)
      Hagiwara K, Fujita H, Kasahara Y, Irisawa Y, Obika S, Kuwahara M.
    • Journal Title

      Mol Biosyst.

      Volume: 11(1) Issue: 1 Pages: 71-76

    • DOI

      10.1039/c4mb00436a

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Polymerase-mediated high-density incorporation of amphiphilic functionalities into DNA: enhancement of nuclease resistance and stability in human serum.2015

    • Author(s)
      Fujita H, Nakajima K, Kasahara Y, Ozaki H, Kuwahara M.
    • Journal Title

      Bioorg Med Chem Lett.

      Volume: 25(2) Pages: 333-6

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Minimal thioflavin T modifications improve visual discrimination of guanine-quadruplex topologies and alter compound-induced topological structures.2014

    • Author(s)
      Kataoka Y, Fujita H, Kasahara Y, Yoshihara T, Tobita S, Kuwahara M.
    • Journal Title

      Anal Chem.

      Volume: 86(24) Issue: 24 Pages: 12078-84

    • DOI

      10.1021/ac5028325

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] CE-SELEX Selection of Base- and Sugar-modified DNA Aptamers : Target Binding Dominated by 2', 4'-BNA/LNA Primer2013

    • Author(s)
      Yuuya Kasahara, et al.
    • Journal Title

      Analytical Chemistry

      Volume: 85(10) Issue: 10 Pages: 4961-4967

    • DOI

      10.1021/ac400058z

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Efficacy of base-modification on target binding of small molecule DNA aptamers2013

    • Author(s)
      Yuri Imaizumi, Yuuya Kasahara, et al.
    • Journal Title

      Journal of the American Chemical Society

      Volume: 135 Issue: 25 Pages: 9412-9419

    • DOI

      10.1021/ja4012222

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] In vitro selection of BNA (LNA) aptamers.2013

    • Author(s)
      Kuwahara M, Obika S.
    • Journal Title

      Artif DNA PNA XNA.

      Volume: 4(2) Issue: 2 Pages: 39-48

    • DOI

      10.4161/adna.25786

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structural and Affinity Analyses of G-Quadruplex DNA Aptamers for Camptothecin Derivatives2013

    • Author(s)
      Hiroto Fujita, Yuri Imaizumi, Yuuya Kasahara, et al.
    • Journal Title

      Pharmaceuticals (Basel)

      Volume: 6 Issue: 9 Pages: 1082-1093

    • DOI

      10.3390/ph6091082

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Application of unnatural nucleicacids and non-canonical nucleic acid structures towards drug discovery and bioanalysis2015

    • Author(s)
      Masayasu Kuwahara
    • Organizer
      2015 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2015)
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2015-12-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Isothermal and visual RNA detection system using enzymatic signal amplification and specific emission of thioflavin-T analog2015

    • Author(s)
      Hiroto Fujita,Yuka Kataoka,Masayasu Kuwahara
    • Organizer
      2015 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2015)
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2015-12-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Thioflavin T analogs as G-quadruplex indicators and their applications to bioanalysis2015

    • Author(s)
      Yuka Kataoka,Hiroto Fujita,Masayasu Kuwahara
    • Organizer
      2015 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2015)
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2015-12-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Capillary electrophoresis-systematic evolution of ligands by exponential enrichment selection using modified nucleic acid libraries2015

    • Author(s)
      Masayasu Kuwahara
    • Organizer
      2015 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2015)
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Xeno-nucleic acid aptamers2015

    • Author(s)
      Masayasu Kuwahara
    • Organizer
      The 1st International Symposium on “Functional Nucleic Acids: From Laboratory to Targeted Molecular Therapy”
    • Place of Presentation
      Perth, Australia
    • Year and Date
      2015-11-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] RNA detection method using a thioflavin-T analog2015

    • Author(s)
      Masayasu Kuwahara, Hiroto Fujita, Yuka Kataoka, Seiji Tobita
    • Organizer
      The 42nd International Symposium on Nucleic Acids Chemistry (ISNAC2015)
    • Place of Presentation
      兵庫・イーグレ姫路
    • Year and Date
      2015-09-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Syntheses and properties of novel Thioflavin T analogs2015

    • Author(s)
      Masayasu Kuwahara
    • Organizer
      Asia 3 Roundtable on Nucleic Acids 2015
    • Place of Presentation
      Suwon, Korea
    • Year and Date
      2015-09-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] キャピラリー電気泳動法を用いた修飾核酸アプタマーのセレクション2015

    • Author(s)
      桑原正靖
    • Organizer
      第66回日本電気泳動学会総会
    • Place of Presentation
      東京・東京工科大学蒲田キャンパス
    • Year and Date
      2015-09-05
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 薬物伝達法への応用に向けた人工核酸アプタマーの開発2015

    • Author(s)
      桑原正靖
    • Organizer
      第31回日本DDS学会学術集会・ワークショップ4・核酸医薬開発の鍵を握るデリバリー技術の新展開
    • Place of Presentation
      東京・京王プラザホテル
    • Year and Date
      2015-07-03
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 医薬・診断薬創製に向けた化学修飾核酸アプタマーのセレクション法開発2015

    • Author(s)
      桑原正靖
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      神戸学院大学
    • Year and Date
      2015-03-28
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] キャピラリー電気泳動セレクション法による血管内皮細胞増殖因子結合性塩基修飾DNAアプタマーのセレクション2015

    • Author(s)
      本田 直渡,笠原 勇矢,萩原 健太,桑原 正靖
    • Organizer
      日本化学会第94春季年会(2015)
    • Place of Presentation
      日本大学理工学部船橋キャンパス/薬学部
    • Year and Date
      2015-03-27
    • Related Report
      2014 Research-status Report
  • [Presentation] オリゴヌクレオチドの鎖長がキャピラリー・ゾーン電気泳動法による標的/核酸アプタマー複合体の分離能に及ぼす影響2015

    • Author(s)
      萩原健太,本田直渡,笠原勇矢,桑原正靖
    • Organizer
      日本化学会第94春季年会(2015)
    • Place of Presentation
      日本大学理工学部船橋キャンパス/薬学部
    • Year and Date
      2015-03-27
    • Related Report
      2014 Research-status Report
  • [Presentation] グアニン四重鎖を標的としたN3位修飾チオフラビンT アナログの創製2015

    • Author(s)
      片岡由佳,藤田博仁,笠原勇矢,桑原正靖
    • Organizer
      日本化学会第94春季年会(2015)
    • Place of Presentation
      日本大学理工学部船橋キャンパス/薬学部
    • Year and Date
      2015-03-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 架橋型ヌクレオチドからなるキセノ核酸の酵素的合成2015

    • Author(s)
      笠井達郎,片岡由佳,藤田博仁,本田直渡,笠原勇矢,小比賀聡,桑原正靖
    • Organizer
      日本化学会第94春季年会(2015)
    • Place of Presentation
      日本大学理工学部船橋キャンパス/薬学部
    • Year and Date
      2015-03-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Modified DNA Aptamers that Bind to Vascular Endothelial Growth Factors2014

    • Author(s)
      Naoto Honda,Kenta Hagiwara,Yuuya Kasahara,Masayasu Kuwahara
    • Organizer
      3rd Asian Chemical Biology Conference (ACBC3)
    • Place of Presentation
      National University of Singapore(NUS), Singapore
    • Year and Date
      2014-12-16
    • Related Report
      2014 Research-status Report
  • [Presentation] Synthesis and Binding Analysis of Thioflavin T Analogs Targeting G-Quadruplex2014

    • Author(s)
      Yuka Kataoka,Hiroto Fujita,Yuuya Kasahara,Toshitada Yoshihara,Seiji Tobita,Masayasu Kuwahara
    • Organizer
      3rd Asian Chemical Biology Conference (ACBC3)
    • Place of Presentation
      National University of Singapore(NUS), Singapore
    • Year and Date
      2014-12-16
    • Related Report
      2014 Research-status Report
  • [Presentation] Selection of DNA aptamers with base modifications using a capillary electrophoresis-based method2014

    • Author(s)
      Yuuya Kasahara,Naoto Honda,Kenta Hagiwara,Masayasu Kuwahara
    • Organizer
      The 41st International Symposium on Nucleic Acids Chemistry 2014 (ISNAC2014)
    • Place of Presentation
      北九州国際会議場,福岡
    • Year and Date
      2014-11-05
    • Related Report
      2014 Research-status Report
  • [Presentation] キャピラリー電気泳動法を用いた人工核酸アプタマーの迅速セレクション法の開発2014

    • Author(s)
      桑原正靖,萩原健太,笠原勇矢
    • Organizer
      日本分析化学会63年会
    • Place of Presentation
      広島大学東広島キャンパス
    • Year and Date
      2014-09-17
    • Related Report
      2014 Research-status Report
  • [Presentation] キャピラリー電気泳動法を用いたタンパク質結合性塩基修飾DNAアプタマーの創製2014

    • Author(s)
      本田直渡,萩原健太,笠原勇矢,桑原正靖
    • Organizer
      日本化学会第8回バイオ関連化学シンポジウム
    • Place of Presentation
      岡山大学津島キャンパス
    • Year and Date
      2014-09-11
    • Related Report
      2014 Research-status Report
  • [Presentation] Modified DNA Aptamer-Based Separation of Small Molecules by Capillary Electrophoresis2014

    • Author(s)
      Yuuya Kasahara,Yurika Watanabe,Masayasu Kuwahara
    • Organizer
      XXI Round Table on Nucleosides, Nucleotides and Nucleic Acids (IRT2014)
    • Place of Presentation
      Poznan, Poland
    • Year and Date
      2014-08-25 – 2014-08-26
    • Related Report
      2014 Research-status Report
  • [Presentation] DNA Aptamers Containing 2'-Deoxy-2'-Fluoroguanosine2014

    • Author(s)
      Kenta Hagiwara,Yuuya Kasahara,Yuuta Irisawa,Masayasu Kuwahara
    • Organizer
      XXI Round Table on Nucleosides, Nucleotides and Nucleic Acids (IRT2014)
    • Place of Presentation
      Poznan, Poland
    • Year and Date
      2014-08-25 – 2014-08-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 人工核酸アプタマー:適合性の向上による標的多様性の拡張2014

    • Author(s)
      本田直渡,笠原勇矢,桒原正靖
    • Organizer
      第16回生命化学研究会
    • Place of Presentation
      KKRホテル熱海,神奈川
    • Related Report
      2013 Research-status Report
  • [Presentation] ポリメラーゼによる高密度修飾ポリヌクレオチド合成とPCR増幅2014

    • Author(s)
      中島康輔, 桒原正靖
    • Organizer
      日本化学会第94春季年会(2014)
    • Place of Presentation
      名古屋大学東山キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] グアニン四重鎖結合性小分子リガンドの創製及び結合特性評価2014

    • Author(s)
      片岡由佳, 藤田博仁, 桒原正靖
    • Organizer
      日本化学会第94春季年会(2014)
    • Place of Presentation
      名古屋大学東山キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 2'-デオキシ-2'-フルオログアノシンを含むDNAアプタマー2014

    • Author(s)
      萩原健太, 笠原勇矢, 藤田博仁, 入澤祐太, 桒原正靖
    • Organizer
      日本化学会第94春季年会(2014)
    • Place of Presentation
      名古屋大学東山キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 非平衡キャピラリー電気泳動法による低分子結合性人工核酸アプタマーの相互作用測定2014

    • Author(s)
      笠原勇矢,渡邉優利花,今泉友里,桒原正靖
    • Organizer
      日本化学会第94春季年会(2014)
    • Place of Presentation
      名古屋大学東山キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] CE-SELEX OF CHIMERIC DNA-BASED APTAMERS2013

    • Author(s)
      Yuuya Kasahara, Yuuta Irisawa, Naoto Honda, Satoshi Obika,Masayasu Kuwahara
    • Organizer
      Frontiers of Single Cell Analysis Conference
    • Place of Presentation
      Stanford University, Stanford, CA, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] NUCLEIC ACID APTAMERS FOR SMALL MOLECULES2013

    • Author(s)
      Hiroto Fujita,Yuri Imaizumi,Yuuya Kasahara,Hiroaki Ozaki,Masayasu Kuwahara
    • Organizer
      Frontiers of Single Cell Analysis Conference
    • Place of Presentation
      Stanford University, Stanford, CA, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] 2',4'-BNA/LNAプライマー依存性キメラ型人工核酸アプタマーの構造と機能2013

    • Author(s)
      笠原勇矢,入澤祐太,本田直渡,森廣邦彦,小比賀聡,桒原正靖
    • Organizer
      第7回バイオ関連化学シンポジウム2013
    • Place of Presentation
      名古屋大学豊田講堂・野依学術交流館
    • Related Report
      2013 Research-status Report
  • [Presentation] 小分子結合性核酸アプタマーの構造と機能2013

    • Author(s)
      藤田 博仁,今泉 友里,笠原 勇矢,尾崎 広明,遠藤 玉樹,桒原 正靖,杉本 直己
    • Organizer
      第7回バイオ関連化学シンポジウム2013
    • Place of Presentation
      名古屋大学豊田講堂・野依学術交流館
    • Related Report
      2013 Research-status Report
  • [Presentation] 含修飾ウラシルライブラリーを用いた高親和性人工核酸アプタマーの創製2013

    • Author(s)
      笠原勇矢,今泉友里,入澤祐太,藤田博仁,本田直渡,桒原正靖
    • Organizer
      第3回CSJ化学フェスタ2013
    • Place of Presentation
      タワーホール船堀,東京
    • Related Report
      2013 Research-status Report
  • [Presentation] Effects of tail strands on the binding activity of G-quadruplex DNA aptamers for small molecules2013

    • Author(s)
      Hiroto Fujita,Yuri Imaizumi,Yuuya Kasahara,Hiroaki Ozaki,Masayasu Kuwahara
    • Organizer
      The 40th International Symposium on Nucleic Acids Chemistry 2013 (ISNAC2014)
    • Place of Presentation
      神奈川大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Switch molecules designed from 2′,4′-BNA aptamers2013

    • Author(s)
      Yuuya Kasahara, Yuuta Irisawa, Naoto Honda, Kunihiko Morihiro, Satoshi Obika, Masayasu Kuwahara
    • Organizer
      The 40th International Symposium on Nucleic Acids Chemistry 2013 (ISNAC2014)
    • Place of Presentation
      神奈川大学
    • Related Report
      2013 Research-status Report
  • [Book] RNA Technologies (Chemical Biology of Nucleic Acids); Progress in Chemically Modified Nucleic Acid Aptamers2014

    • Author(s)
      M. Kuwahara
    • Publisher
      Springer
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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