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Biofilm formation mechanism by intercellular signaling molecule of environmental pathogen

Research Project

Project/Area Number 25350968
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biomolecular chemistry
Research InstitutionOsaka City University

Principal Investigator

Nishiuchi Yukiko  大阪市立大学, 大学院医学研究科, 助教 (00333526)

Co-Investigator(Kenkyū-buntansha) NISHIUCHI Yuji  大阪市立大学, 大学院医学研究科, 学外研究員 (30132814)
MATSUMOTO Sohkichi  新潟大学, 大学院医歯学総合研究科, 教授 (30244073)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords非結核性抗酸菌 / バイオフィルム / トリ型結核菌 / グリコペプチドリピド / 低酸素 / トレハロースジマイコレート / 細胞間情報伝達物質 / Mycobacterium avium
Outline of Final Research Achievements

The incidence of pulmonary Mycobacterium avium complex (MAC) disease is increasing in Japan. This disease is generally developed by inhalation of MAC organisms occurring in the environment. MAC forms biofilm in the environment and hosts, and the biofilm can function to shelter MAC itself. Thus, the formed biofilm makes it difficult to eliminate MAC organisms from the infection sources and hosts. However, the biology and the regulatory mechanism of biofilm formation of MAC still remain unknown. The aim of this research is to elucidate these pending issues with the biofilm formation of MAC. We were able to demonstrate that the ambient hypoxia and eutrophic conditions promote MAC biofilm formation, and that glycolipids existing on the envelope of MAC, such as glycopeptidolipid, trehalose dimycolate, and trehalose monomycolate, are greatly involved in the formation of biofilm. Based on these findings, we are trying to find out measures that can effectively disinfect MAC organisms.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (23 results)

All 2016 2015 2014 2013 Other

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (16 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Book (1 results)

  • [Journal Article] A new screen for tuberculosis drug candidates utilizing a luciferase-expressing recombinant Mycobacterium bovis Bacillus Calmette-Guerin2015

    • Author(s)
      Ozeki Y, Igarashi M, Doe M, Tamaru A, Kinoshita N, Ogura Y, Iwamoto T, Sawa R, Umekita M, Enany S, Nishiuchi Y, Osada-Oka M, Hayashi T, Niki M, Tateishi Y, Hatano M, Matsumoto S.
    • Journal Title

      Plos One

      Volume: 10

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 抗酸菌およびそのバイオフィルムに対する次亜塩素酸ナトリウムと二酸化塩素ガス溶存液の殺菌効果2015

    • Author(s)
      西内由紀子 田丸亜貴 戸谷孝洋
    • Journal Title

      日本環境感染学会誌

      Volume: 印刷中

    • NAID

      130005102256

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] パイやカップ麺に含まれる飽和脂肪酸およびトラン ス脂肪酸の分析2015

    • Author(s)
      馬場 恒子, 野阪 美貴子, 西内 由紀子
    • Journal Title

      J. Fac. Human Sci. Kobe Shoin Women's Univ.

      Volume: 4 Pages: 1-9

    • NAID

      120005568815

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 1.Direct detection of Mycobacterium avium in environmental water and scale samples by loop-mediated isothermal amplification.2014

    • Author(s)
      Nishiuchi Y, Tamaru A, Suzuki Y, Kitada S, Maekura R, Tateishi Y, Niki M, Ogura H, Matsumoto S
    • Journal Title

      J Water Health.

      Volume: 12 Issue: 2 Pages: 211-219

    • DOI

      10.2166/wh.2013.007

    • Related Report
      2014 Research-status Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] ntra-subspecies sequence variability of the MACPPE12 gene in Mycobacterium avium subsp. Hominissuis2014

    • Author(s)
      Iwamoto T, Arikawa K, Nakajima C, Nakanishi N, Nishiuchi Y, Yoshida S, Tamaru A,Tamura Y, Hoshino Y, Yoo H, Park YK, Saito H, Suzuki Y
    • Journal Title

      Infect. Genet. Evolu.

      Volume: 21 Pages: 476-483

    • DOI

      10.1016/j.meegid.2013.08.010

    • Related Report
      2014 Research-status Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 抗酸菌の細菌学的特徴と病原性2014

    • Author(s)
      西内由紀子 松本壮吉
    • Journal Title

      感染症内科

      Volume: 2 Pages: 8-14

    • Related Report
      2013 Research-status Report
  • [Presentation] Pathogenic slow growers ―environmental niches and biofilm formation―2016

    • Author(s)
      Yukiko Nishiuchi
    • Organizer
      ASM Microbe 2016
    • Place of Presentation
      Boston, USA
    • Year and Date
      2016-06-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 長期観察している抗酸菌症患者から得られた経時的分離株の遺伝子解析2016

    • Author(s)
      有川健太郎、岩本朋忠、西内由紀子
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪国際交流センター(大阪府大阪市)
    • Year and Date
      2016-03-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] 非結核性抗酸菌のバイオフィルム形成におけるGlycopeptidolipidの役割2016

    • Author(s)
      西内由紀子、戸谷孝洋、立石善隆、金子幸弘、松本壮吉
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪国際交流センター(大阪府大阪市)
    • Year and Date
      2016-03-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] Mycobacterium avium の系統分岐に伴う大規 模なゲノム構造の変化2016

    • Author(s)
      岩本 朋忠,矢野 大和,西内 由紀子,有川 健太郎,中島 千絵,鈴木 定彦,丸山 史人
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪国際交流センター(大阪府大阪市)
    • Year and Date
      2016-03-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] ルシフェラーゼ発現リコンビナ ントBCG による新規結核薬の迅速スクリーニング系の確立 と実践2016

    • Author(s)
      尾関 百合子,山口 雄大,Shymaa Enany,五十嵐 雅之,西内由紀子,岡 真優子,岩本 朋忠,小椋 義俊,林 哲也,立石 善隆,西山 晃史,松本 壮吉
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪国際交流センター(大阪府大阪市)
    • Year and Date
      2016-03-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] Characteristics of nontuberculous mycobacterial biofilm formation2016

    • Author(s)
      Yukiko NIshiuchi, Takahiro Totani, Yoshitaka Tateishi, Yukihiro Kaneko, Sohkichi Matsumoto
    • Organizer
      US-JAPAN Cooperatie Medical Science Program
    • Place of Presentation
      Bethesda, USA
    • Year and Date
      2016-01-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 浴室より分離された Mycobacterium avium subsp. hominissuisのバイオフィルム形成における環境因子の検討2015

    • Author(s)
      西内 由紀子 , 前倉 亮治
    • Organizer
      第46回 結核・非定型抗酸菌症治療研究会
    • Place of Presentation
      笹川記念会館 (東京都港区)
    • Year and Date
      2015-12-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] Diversity in Mycobacteria: Distribution, host, morphology and genome2015

    • Author(s)
      西内由紀子
    • Organizer
      第30回 日本微生物生態学会
    • Place of Presentation
      土浦亀城プラザ (茨城県土浦市)
    • Year and Date
      2015-10-17
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] バイオフィルム形成と環境中気体組成2015

    • Author(s)
      西内由紀子、戸谷孝洋、金子幸弘、松本壮吉
    • Organizer
      第29回 バイオフィルム学会
    • Place of Presentation
      ホテル竹島(愛知県蒲郡市)
    • Year and Date
      2015-07-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] 環境から分離した非結核性抗酸菌のバイオフィルム形成促進因子2015

    • Author(s)
      西内由紀子、金子幸弘、松本壮吉
    • Organizer
      第90回日本結核病学会
    • Place of Presentation
      長崎ブリックホール( 長崎県・長崎市)
    • Year and Date
      2015-03-27 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] 結核免疫に及ぼす血清脂質の影響2015

    • Author(s)
      金子幸弘、井上学、仁木満美子、 西内由紀子、松本壮吉、掛屋弘
    • Organizer
      第90回日本結核病学会
    • Place of Presentation
      長崎ブリックホール( 長崎県・長崎市)
    • Year and Date
      2015-03-27 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] M. aviumのバイオフィルム形成時の特徴2015

    • Author(s)
      戸谷孝洋、西内由紀子、金子幸弘、松本壮吉
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      長良川国際会議場( 岐阜県・岐阜市)
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] 非結核性抗酸菌のバイオフィルム形成時の特徴2014

    • Author(s)
      戸谷孝洋、西内由紀子、北中博美、金子幸弘、松本壮吉
    • Organizer
      第28回Bacterial Adherence & Biofilm学術集会
    • Place of Presentation
      都市センターホテル ( 東京都・千代田区)
    • Year and Date
      2014-07-09
    • Related Report
      2014 Research-status Report
  • [Presentation] 非結核性抗酸菌のバイオフィルム 形成解析2014

    • Author(s)
      戸谷孝洋、西内由紀子、松本壮吉
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      タワーホール船堀
    • Related Report
      2013 Research-status Report
  • [Presentation] 非結核性抗酸菌のバイオフィルム形成因子

    • Author(s)
      西内由紀子、戸谷孝洋、松本壮吉
    • Organizer
      第27回 Bacterial adherence & Biofilm 学術集会
    • Place of Presentation
      東京ガーデンパレス
    • Related Report
      2013 Research-status Report
  • [Presentation] 非結核性抗酸菌 Mycobacterium aviumのバイオフィルム形成解析

    • Author(s)
      戸谷孝洋、西内由紀子
    • Organizer
      第66回日本細菌学会関西支部総会
    • Place of Presentation
      大阪大学微生物研究所
    • Related Report
      2013 Research-status Report
  • [Book] 肺MAC症診療 Up to Date /非結核性抗酸菌とバイオフィルム-お風呂は感染源か?2013

    • Author(s)
      西内由紀子
    • Total Pages
      272
    • Publisher
      南江堂
    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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