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Development of genetically-encoded multi-color ATP sensors for clarification of energy metabolism.

Research Project

Project/Area Number 25350970
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biomolecular chemistry
Research InstitutionWaseda University

Principal Investigator

KITAGUCHI TETSUYA  早稲田大学, 総合研究機構, 主任研究員(研究院准教授) (60432374)

Co-Investigator(Kenkyū-buntansha) 新井 敏  早稲田大学, 総合研究機構, 次席研究員(研究院講師) (70454056)
坪井 貴司  東京大学, 大学院総合文化研究科, 准教授 (80415231)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
KeywordsGFP / ATP / 可視化
Outline of Final Research Achievements

Adenosine triphosphate (ATP) is an energy currency for living organisms. Therefore, we are able to clarify the mechanism of energy metabolism by visualizing ATP dynamics in the living cells. To achieve this objective, we developed a genetically-encoded fluorescent indicator for ATP. These indicators are single-fluorescent protein (FP) based, therefore change their intensity respond to ATP. We employed a green FP variant, Citrine as transmitter domain and a subunit of ATP-binding region on the bacterial FoF1-ATP synthase as sensing domain. Likewise, we employed blue FP and mApple to develop blue or red emitted indicators, respectively. Based on these indicators, we have monitored ATP dynamics in living cells by multi-color imaging.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (14 results)

All 2016 2015 2014 2013

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (8 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] 蛍光・発光プローブ2016

    • Author(s)
      北口哲也
    • Journal Title

      光アライアンス

      Volume: 27 Pages: 15-19

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Low glucose-induced ghrelin secretion is mediated by ATP-sensitive potassium channel.2015

    • Author(s)
      Manami Oya, Tetsuya Kitaguchi, Kazuki Harada, Rika Numano, Takahiro Sato, Masayasu Kojima and Takashi Tsuboi.
    • Journal Title

      J Endocrinol

      Volume: 226 Issue: 1 Pages: 25-34

    • DOI

      10.1530/joe-15-0090

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Genetically-encoded yellow fluorescent cAMP indicator with an expanded dynamic range for dual-color imaging.2014

    • Author(s)
      Haruki Odaka, Satoshi Arai, Takafumi Inoue and Tetsuya Kitaguchi.
    • Journal Title

      PLoS One

      Volume: 9 Issue: 6 Pages: e100252-e100252

    • DOI

      10.1371/journal.pone.0100252

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Vesicular nucleotide transporter is involved in ATP storage of secretory lysosomes in astrocytes.2013

    • Author(s)
      Manami Oya
    • Journal Title

      Biochemical Biophysical Research Communications.

      Volume: 438 Issue: 1 Pages: 145-151

    • DOI

      10.1016/j.bbrc.2013.07.043

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] RGBカラーの蛍光ATPセンサーが切り拓くエネルギー代謝研究2016

    • Author(s)
      新井敏、伊藤寿朗、坪井貴司、北口哲也
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] Spatiotemporal imaging and quantitative analysis of subcellular ATP using RGB-colorful fluorescent protein based indicators.2016

    • Author(s)
      Satoshi Arai, Hideki Itoh, Thankiah Sudhaharan, E. Birgitte Lane and Tetsuya Kitaguchi.
    • Organizer
      第54回日本生物物理学会
    • Place of Presentation
      つくば
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Visualization of subcellular ATP dynamics in Arabiodopsis protoplasts with an intensiometric fluorescent protein-based indicator.2015

    • Author(s)
      Satoshi Arai, Liang-Sheng Looi, Wan-Yi Wee, Toshiro Ito and Tetsuya Kitaguchi.
    • Organizer
      第38回分子生物学会
    • Place of Presentation
      兵庫県神戸市 神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Improvement of genetically-encoded yellow fluorescent cAMP indicator for dual-color imaging.2014

    • Author(s)
      Haruki Odaka, Satoshi Arai, Takafumi Inoue and Tetsuya Kitaguchi.
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] An intensiometric fluorescent protein-based indicator visualizes ATP dynamics in intracellular compartments.2014

    • Author(s)
      Satoshi Arai, Rokus Gyorgy Kriszt, Michael Raghunath and Tetsuya Kitaguchi.
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Vesicular nucleotide transporter regulates ATP storage in secretory lysosome in astrocyte.2014

    • Author(s)
      Takashi Tsuboi, Manami Oya and Tetsuya Kitaguchi.
    • Organizer
      第91回日本生理学会大会
    • Place of Presentation
      鹿児島
    • Related Report
      2013 Research-status Report
  • [Presentation] タンパク質の構造変化を光情報に変換するバイオセンサーの創出2013

    • Author(s)
      新井敏、北口哲也
    • Organizer
      第62回高分子討論会
    • Place of Presentation
      金沢
    • Related Report
      2013 Research-status Report
  • [Presentation] Development of intensiometric fluorescent indicators for sensing the dynamics of intracellular ATP.2013

    • Author(s)
      Satoshi Arai, Manami Oya, Takashi Tsuboi and Tetsuya Kitaguchi.
    • Organizer
      第36回分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
  • [Patent(Industrial Property Rights)] 「リガンド蛍光センサータンパク質とその使用 」2016

    • Inventor(s)
      北口哲也、坪井貴司、上田宏
    • Industrial Property Rights Holder
      北口哲也、坪井貴司、上田宏
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-12-02
    • Related Report
      2016 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] ATP蛍光センサータンパク質2015

    • Inventor(s)
      北口哲也、新井敏、坪井貴司
    • Industrial Property Rights Holder
      北口哲也、新井敏、坪井貴司
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-237524
    • Filing Date
      2015-12-04
    • Related Report
      2015 Research-status Report

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Published: 2014-07-25   Modified: 2021-01-27  

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