| Project/Area Number |
25350973
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Suntory Foundation for Life Sciences |
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Principal Investigator |
Murata Yoshiko 公益財団法人サントリー生命科学財団, その他部局等, 研究員 (60256047)
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| Co-Investigator(Kenkyū-buntansha) |
NAMBA KOSUKE 北海道大学, 理学研究科, 准教授 (50414123)
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| Co-Investigator(Renkei-kenkyūsha) |
KIMURA HIROYUKI 京都大学, 放射線同位元素総合センター, 助教 (50437240)
YAMAGAKI TOHRU 公益財団法人サントリー, 生物有機科学研究所, 主席研究員 (40313209)
TAKAHASHI TOSHIO 公益財団法人サントリー, 生物有機科学研究所, 研究員 (20390792)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 鉄 / ニコチアナミン / 植物性食物 / キレート化合物 / 小腸 / 鉄代謝 / 小腸吸収 / トランスポーター / caco2細胞 |
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| Outline of Final Research Achievements |
Nicotianamine (NA) plays an important role as an iron chelator for transporting iron inside the plant. Plant foods such as beans and broccoli contain a large amount of NA. For elucidating the effects of NA upon iron absorption in human, we carried out in vitro assays for examining the uptake of NA and its 59Fe complex using caco-2 monolayer cells, which were widely used as a model for human intestinal absorption. The results revealed the NA-dependent increase of iron efflux, further suggesting that the Fe-NA complex transports iron to the basal side (the blood vessel side) via caco-2 cells. For in vivo assays showed that oral Fe-NA administrations to mice enhance the iron content of lung and stomach after two hours, and skeleton muscle after five hours. The difference in iron concentrations between iron only and Fe-NA complex by oral administration was significant in the mouse tissues, further suggesting that NA is implicated in iron trafficking in the human intestine.
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