Excitatory synaptic transmission is regulated by the number of regional astrocytes in single cultured hippocampal neuron

• Project/Area Number: 25350988
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Basic / Social brain science
• Research Institution: Fukuoka University
• Principal Investigator: Katsurabayashi Shutaro 福岡大学, 薬学部, 准教授 (50435145)
• Co-Investigator(Kenkyū-buntansha): KIMURA YASUO 東京工科大学, 工学部, 教授 (40312673) ; MA TENG 東北大学, 電気通信研究所, 助教 (10734543)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: アストロサイト / シナプス伝達 / グリア

Outline of Final Research Achievements

Neural circuit is composed of mainly neuron and glial cell. Astrocyte is a major glial cell which plays key roles in brain function including formation and maturation of synapses. In this study, we utilized primary co-culture system of autaptic single hippocampal neuron with dot-cultured cortical astrocytes, where experimentally modified the density of astrocytes. Excitatory synaptic transmission was decreased by lower density of astrocytes (the number of astrocytes = 1-5), which was accompanied by a decrease of readily releasable pool of synaptic vesicles but not of the vesicular release probability. Distribution analysis of synaptic puncta stained with FM styryl dye and VGLUT-1 antibody revealed that a proportion of presynaptically silent synapses on dendrites was increased by a small density of astrocytes. These findings indicate that synaptic transmission and a position of presynaptically active synapse are varied by the number of astrocytes surrounding neuron.

Research Products

• [Journal Article] Overexpression of Swedish mutant APP in aged astrocytes attenuates excitatory synaptic transmission. (2016)
  • Author(s): Katsurabayashi S, Kawano H, Ii M, Nakano S, Tatsumi C, Kubota K, Takasaki K, Mishima K, Fujiwara M, Iwasaki K.
  • Journal Title: Physiological Reports Volume: 4 Issue: 1 Pages: e12665-e12665
  • DOI: 10.14814/phy2.12665
  • Peer Reviewed / Open Access
• [Journal Article] Rheb activation disrupts spine synapse formation through accumulation of syntenin in tuberous sclerosis complex (2015)
  • Author(s): Hiroko Sugiura, Shin Yasuda, Shutaro Katsurabayashi, Hiroyuki Kawano, Kentaro Endo, Kotaro Takasaki, Katsunori Iwasaki, Masumi Ichikawa, Toshiyuki Kobayashi, Okio Hino and Kanato Yamagata
  • Journal Title: Nature Communications Volume: 6 Issue: 1 Pages: 6842-6842
  • DOI: 10.1038/ncomms7842
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Activation of Rheb, but not of mTORC1, impairs spine synapse morphogenesis in tuberous sclerosis complex. (2014)
  • Author(s): Yasuda S, Sugiura H, Katsurabayashi S, Shimada T, Tanaka H, Takasaki K, Iwasaki K, Kobayashi T, Hino O, Yamagata K.
  • Journal Title: Sci Rep. Volume: 4 Issue: 1 Pages: 5155-5155
  • DOI: 10.1038/srep05155
  • Peer Reviewed / Open Access
• [Journal Article] Effect of Yokukansan on sleep disturbance in a rat model of cerebrovascular dementia (2014)
  • Author(s): Nagao M, Takasaki K, Nogami A, Hirai Y, Moriyama H, Uchida N, Kubota K, Katurabayashi S, Mishima K, Nishimura R, Iwasaki K
  • Journal Title: Traditional & Kampo Medicine Volume: 1 (2) Issue: 2 Pages: 19-26
  • DOI: 10.1002/tkm2.1008
  • Peer Reviewed
• [Presentation] Distribution of presynaptically active synapses is regulated by the number of regional astrocytes in single cultured hippocampal neuron (2016)
  • Author(s): Hideto Okuda, Takashi Hoshiyama, Saori Iwamoto, Kotomi Takeda, Kaori Kubota, Takuya Watanabe, Shutaro Katsurabayashi, Katsunori Iwasaki
  • Organizer: The 3rd RIEC International Symposium on Brain Functions and Brain Computer
  • Place of Presentation: 東北大学
  • Year and Date: 2016-02-23
  • Int'l Joint Research
• [Presentation] Relationship between density of local astrocytes and excitatory synaptic transmission in vitro (2015)
  • Author(s): Hideto Okuda, Takashi Hoshiyama, Saori Iwamoto, Kotomi Takeda, Shutaro Katsurabayashi, Katsunori Iwasaki
  • Organizer: 生理研シナプス研究会
  • Place of Presentation: 生理学研究所
  • Year and Date: 2015-12-02
• [Presentation] アストロサイトの培養環境制御によるシナプス伝達のパラダイム (2014)
  • Author(s): 星山貴志、岩本彩緒里、武田琴水、青沼有紀、庭野道夫、野田百美、桂林秀太郎、岩崎克典
  • Organizer: 第67回日本薬理学会西南部会
  • Place of Presentation: 北九州
  • Year and Date: 2014-11-23
• [Presentation] 長期培養型アストロサイトによるシナプス伝達の低下 (2014)
  • Author(s): 桂林秀太郎
  • Organizer: 第24回日本病態生理学会
  • Place of Presentation: 北九州
  • Year and Date: 2014-08-09
  • Invited
• [Presentation] アストロサイト領域の条件変化とシナプス伝達 (2014)
  • Author(s): 桂林秀太郎、河野洋幸、岩崎克典
  • Organizer: 第20回BMIRC研究会【センサーと神経工学】
  • Place of Presentation: 北九州
  • Year and Date: 2014-08-05
  • Invited
• [Presentation] シナプス伝達を得るために必要なアストロサイト環境の模索 (2014)
  • Author(s): 桂林秀太郎
  • Organizer: 第16回ブレインサイエンス研究会
  • Place of Presentation: 沖縄
  • Year and Date: 2014-06-28 – 2014-06-29
• [Presentation] アストロサイトによるシナプス伝達の修飾 (2014)
  • Author(s): 桂林秀太郎
  • Organizer: 琉球大学医学部分子解剖学セミナー
  • Place of Presentation: 沖縄
  • Year and Date: 2014-06-27
  • Invited
• [Presentation] アルツハイマー病新規治療戦略を指向した異常老化アストロサイトに関する研究 (2014)
  • Author(s): 河野洋幸、桂林秀太郎、窪田香織、高崎浩太郎、三島健一、藤原道弘、岩崎克典
  • Organizer: 第16回七隈アルツハイマー病・パーキンソン病研究会（福岡）
  • Place of Presentation: 福岡
  • Year and Date: 2014-04-26
• [Presentation] アストロサイト数の制御に伴うシナプス伝達の変化 (2014)
  • Author(s): 桂林 秀太郎
  • Organizer: 生体制御・創薬研究ワークショップ in 鹿児島
  • Place of Presentation: 鹿児島
  • Invited
• [Presentation] The number of astrocytes is critical for the synaptic transmission in a single cultured neuron (2014)
  • Author(s): 桂林 秀太郎
  • Organizer: The 2nd RIEC International Symposium on Brain Functions and Brain Computer
  • Place of Presentation: 仙台
  • Invited