| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Neural circuit is composed of mainly neuron and glial cell. Astrocyte is a major glial cell which plays key roles in brain function including formation and maturation of synapses. In this study, we utilized primary co-culture system of autaptic single hippocampal neuron with dot-cultured cortical astrocytes, where experimentally modified the density of astrocytes. Excitatory synaptic transmission was decreased by lower density of astrocytes (the number of astrocytes = 1-5), which was accompanied by a decrease of readily releasable pool of synaptic vesicles but not of the vesicular release probability. Distribution analysis of synaptic puncta stained with FM styryl dye and VGLUT-1 antibody revealed that a proportion of presynaptically silent synapses on dendrites was increased by a small density of astrocytes. These findings indicate that synaptic transmission and a position of presynaptically active synapse are varied by the number of astrocytes surrounding neuron.
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