The analysis of cholinergic modulations of the visual responsiveness

• Project/Area Number: 25350991
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Basic / Social brain science
• Research Institution: National Center of Neurology and Psychiatry (2015); The Institute of Physical and Chemical Research (2013-2014)
• Principal Investigator: Sohya Kazuhiro 国立研究開発法人国立精神・神経医療研究センター, 神経研究所 疾病研究第三部, 室長 (80415207)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 二光子レーザー走査型顕微鏡 / カルシウムイメージング / 大脳皮質視覚野 / 方位選択性 / in vivo / 視覚応答 / 蛍光タンパク質 / 抑制性ニューロン / 二光子励起 / GFP / 大脳皮質初期視覚野 / GABAニューロン / 覚醒脳 / ニューロンクラスター / コリン作動性ニューロン

Outline of Final Research Achievements

In this study, I revealed that the effect of awakening is mediated mainly through the activation of GABAergic neurons, which modifies the visual responses of excitatory neurons. Also I developed the new method of GABAergic neuron activity in 3D space. Then I revealed that GABAergic interneurons form clusters to make their inhibitory function.

Research Products

• [Journal Article] 3D Clustering of GABAergic Neurons Enhances Inhibitory Actions on Excitatory Neurons in the Mouse Visual Cortex. (2014)
  • Author(s): Ebina T, Sohya K, Imayoshi I, Yin ST, Kimura R, Yanagawa Y, Kameda H, Hioki H, Kaneko T, Tsumoto T.
  • Journal Title: Cell Rep Volume: 9(5) Issue: 5 Pages: 1896-1907
  • DOI: 10.1016/j.celrep.2014.10.057
  • Peer Reviewed
• [Journal Article] Curtailing effect of awakening on visual responses of cortical neurons by cholinergic activation of inhibitory circuits. (2014)
  • Author(s): Kimura R, Safari MS, Mirnajafi-Zadeh J, Kimura R, Ebina T, Yanagawa Y, Sohya K, Tsumoto T.
  • Journal Title: The Journal of Neuroscience. Volume: 34 Issue: 30 Pages: 10122-10133
  • DOI: 10.1523/jneurosci.0863-14.2014
  • Peer Reviewed / Open Access
• [Journal Article] Reciprocal Homosynaptic and heterosynaptic long-term plasticity of corticogeniculate projection neurons in layer VI of the mouse visual cortex. (2013)
  • Author(s): Arami MK, Sohya K, Sarihi A, Jiang B, Yanagawa Y, Tsumoto T.
  • Journal Title: The Journal of Neuroscience. Volume: 33 Issue: 18 Pages: 7787-7798
  • DOI: 10.1523/jneurosci.5350-12.2013
  • Peer Reviewed
• [Presentation] マウス大脳皮質視覚野におけるGABA作動性ニューロンクラスターの機能的意義 (2014)
  • Author(s): 蝦名 鉄平、惣谷 和広、今吉 格、Yin Shu-Ting、木村 塁、柳川 右千夫、亀田 浩司、日置 寛之、金子 武嗣、津本 忠治
  • Organizer: 第３７回神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-09-11
• [Presentation] 二光子顕微鏡を用いた麻酔、覚醒状態での視覚応答特性の比較 (2013)
  • Author(s): 木村 塁、惣谷 和広、尾関 宏文、蝦名 鉄平、柳川 右千夫、津本 忠治
  • Organizer: Neuro2013、第36回日本神経科学大会第56回日本神経化学会大会第23回日本神経回路学会大会合同大会
  • Place of Presentation: 国立京都国際会館
• [Remarks] 大脳皮質の神経細胞はなぜ多いのか、その理由を発見
  • URL: http://www.riken.jp/pr/press/2014/20141121_1/
• [Remarks] 覚醒や注意が感覚を鋭くする脳回路を解明
  • URL: http://www.riken.jp/pr/press/2014/20140723_1/