| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
To synthesize uniform single nano-crystal in the protein (ferritin) cavity, we have tried mutagenesis of the ferritin subunit. Ferritin molecule is consisted in 24 of H and L subunits. H-subunit has an iron oxidation site and H-ferritin which has 24 H-subunits rapidly produces magnetite nanoparticle in the cavity and thus nanoparticles are polycrystalline. H- ferritin is easy to make coagulation during synthesis. L-ferritin which has 24 L-subunits can also produce magnetite nanoparticles even it has no iron oxidation site, whereas efficiency is lower than H-ferritin. Then we have introduced iron oxidation site in the L-subunit and tried to express this mutant with L subunits simultaneously and construct a chimera-ferritin molecule. However, the oxidation activity was similar as the wild type. Then, we have tried to optimize synthesis condition using L-ferritin and found single nano-crystal can be obtained effectively by incubating L- ferritin at 60 degrees with keeping pH at 8.5.
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