| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Outline of Final Research Achievements |
We studied on performance of the waveguide-mode sensor as a biosensor. Various triethoxysilane derivatives were synthesized to modify the surface of sensing chip, and the sensing interface for the sensor was fabricated to immobilize anti-leptin antibody on the modified chip. The detection limit of leptin was examined with modified sensing chips using various triethoxysilane derivatives, and the detection limits of leptin were several tens of ng/mL in PBS, 100 ng/mL in human serum, and several ng/mL in PBS with secondary antibody enhancement, respectively. The fabricated sensing interface showed an excellent potential against non-specific adsorption of proteins. These results suggests that the waveguide-mode sensor has potential for practical use as a biosensor.
|
