Studies on Fabrication of Immunosensing Interface
| Project/Area Number |
25390022
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Nanomaterials chemistry
|
|---|
| Research Institution | National Institute of Advanced Industrial Science and Technology |
|---|
Principal Investigator |
Tanaka Mutsuo 国立研究開発法人産業技術総合研究所, 健康工学研究部門, グループ長 (70344108)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | 表面修飾材料 / 抗原抗体反応 / バイオセンサー / 導波モードセンサー / 非特異吸着抑制 / 抗体固定化 / イムノセンシング / ガラス基板 / シラン化合物 / 抗体固定化材料 / 非特異吸着抑制材料 |
|---|
| Outline of Final Research Achievements |
We studied on performance of the waveguide-mode sensor as a biosensor. Various triethoxysilane derivatives were synthesized to modify the surface of sensing chip, and the sensing interface for the sensor was fabricated to immobilize anti-leptin antibody on the modified chip. The detection limit of leptin was examined with modified sensing chips using various triethoxysilane derivatives, and the detection limits of leptin were several tens of ng/mL in PBS, 100 ng/mL in human serum, and several ng/mL in PBS with secondary antibody enhancement, respectively. The fabricated sensing interface showed an excellent potential against non-specific adsorption of proteins. These results suggests that the waveguide-mode sensor has potential for practical use as a biosensor.
|
Report
(4 results)
Research Products
(12 results)
