Controlling the higher-order structure of genomic DNA in relation to its genetic activity
| Project/Area Number |
25390038
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nanobioscience
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
YOSHIKAWA Yuko 立命館大学, 総合科学技術研究機構, 教授 (80291871)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 長鎖DNA / 単一分子観察 / 高次構造 / 二重鎖切断 / DNA損傷 / 一分子イメージング / 長鎖DNAの高次構造 / 構造機能相関 / １分子イメージング |
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| Outline of Final Research Achievements |
Through the use of single-molecule observation with fluorescence microscopy, we studied the higher-order structural change of a giant DNA molecule. Results obtained are as follows.
(1) The effects of linear, branched and cyclic polyamines on the higher-order structure of giant DNA were examined. These polyamines showed distinct activities, thus highlighting the importance of polyamine backbone structure. (2) The protective effect of ascorbic acid (AsA) against double-strand breaks in DNA was evaluated. Samples were exposed to three different irradiations: visible light, gamma-ray and ultrasound. It was found that AsA had almost no protective effect against the damage caused by ultrasound.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles.2014
Author(s)
Okada K, Hidese R, Fukuda W, Niitsu M, Takao K, Horai Y, Umezawa N, Higuchi T, Oshima T, Yoshikawa Y, Imanaka T, Fujiwara S
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Journal Title
J.Bacteriol.
Volume: 196
Issue: 10
Pages: 1866-1876
DOI
Related Report
Peer Reviewed
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[Journal Article] Chromatin compaction protects genomic DNA from radiation damage.2013
Author(s)
Takata, H., Hanafusa, T., Mori, T., Shimura, M., Iida,Y., Ishikawa, K., Yoshikawa, K., Yoshikawa, Y., Maeshima, K.
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Journal Title
PLOS ONE
Volume: 8
Issue: 10
Pages: e75622-e75622
DOI
Related Report
Peer Reviewed / Open Access
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