Development of highly sensitive affinity biosensor with interdigitated microelectrodes
| Project/Area Number |
25390050
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied materials
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| Research Institution | Tokyo University of Marine Science and Technology |
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Principal Investigator |
OHNUKI Hitoshi 東京海洋大学, 学術研究院, 准教授 (60223898)
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| Co-Investigator(Renkei-kenkyūsha) |
ENDO. Hideaki 東京海洋大学, 学術研究院, 教授 (50242326)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 電気化学インピーダンス / バイオセンサ / 免疫センサ / くし形電極 / アフィニティ反応 / 電気化学インピーダンス法 |
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| Outline of Final Research Achievements |
Affinity biosensor based on electrochemical impedance spectroscopy possesses attractive advantages. But the small signal intensity limits the detection performance. In this study, we examined several attempts to increase the intensity using the interdigitated microelectrodes.
First, we intended to optimize the distribution of electric field on the electrode. The edge lines of interdigitated electrodes were covered with SiO2 layer to locate the electric field on the central area of electrode. However, the sensing performance was not as good as normal ones. The result suggested that a disordered surface may increase the performance. To examine this idea, we employed a mixed SAM. One of the SAM molecule has a short and inactive terminal group, and the domains act as pin-hole defects. It was found that the biosensor contains the 75 % areal pin-holes significantly improved the sensing performance. We proposed a model to increase sensing performance by the pin-holes.
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Report
(4 results)
Research Products
(13 results)
