• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Redox property of water-oxydizing catalyst Mn cluster in photosynthesis

Research Project

Project/Area Number 25410009
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Physical chemistry
Research InstitutionNagoya University

Principal Investigator

Kato Yuki  名古屋大学, 理学(系)研究科(研究院), 助教 (10376634)

Co-Investigator(Renkei-kenkyūsha) NOGUCHI Takumi  名古屋大学, 大学院理学研究科, 教授 (60241246)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords光合成 / 水分解 / 酸素発生 / 電子伝達 / 光化学系 / 酸化還元電位 / 分光電気化学 / FTIR
Outline of Final Research Achievements

Photosystem II (PSII) extracts electrons from water at a Mn4CaO5 cluster using light energy and then transfers them to two plastoquinones, the primary quinone electron acceptor QA and the secondary quinone QB. This forward electron transfer is an essential process in light energy conversion. Meanwhile, backward electron transfer is also significant in photoprotection. Modulation of the redox potential (Em) gap of QA and QB mainly regulates the forward and backward electron transfers in PSII. However, the full scheme of electron transfer regulation remains unresolved due to the unknown Em value of QB. In this work, the Em value of QB reduction was measured directly using spectroelectrochemistry in combination with light-induced Fourier transform infrared difference spectroscopy. The result explains the mechanism of PSII photoprotection with an impaired Mn4CaO5 cluster, in which a large decrease in the Em gap between QA and QB promotes rapid charge recombination via QA-.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (30 results)

All 2016 2015 2014 2013

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 1 results,  Acknowledgement Compliant: 3 results) Presentation (25 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Book (1 results)

  • [Journal Article] Mutation-induced perturbation of the special pair P840 in the homodimeric reaction center in green sulfur bacteria2016

    • Author(s)
      C. Azai, Y. Sano, Y. Kato, T. Noguchi and H. Oh-oka
    • Journal Title

      Sci. Rep.

      Volume: ー Issue: 1 Pages: 19878-19878

    • DOI

      10.1038/srep19878

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Redox potential of the terminal quinone electron acceptor QB in photosystem II reveals the mechanism of electron-transfer regulation2016

    • Author(s)
      Y. Kato, R. Nagao, T. Noguchi
    • Journal Title

      Proc. Natl. Acad. Sci. U.S.A.

      Volume: 113 Issue: 3 Pages: 620-625

    • DOI

      10.1073/pnas.1520211113

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] FTIR spectroelectrochemistry combined with a light-induced difference technique: Application to the iron-quinone electron acceptor in photosystem II2016

    • Author(s)
      Yuki Kato, Takumi Noguchi
    • Journal Title

      Biomedical Spectroscopy and Imaging

      Volume: -

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Long-range interaction between the Mn_4CaO_5-cluster and the non-hemeiron center in photosystem II as revealed by FTIR spectroelectrochemistry2014

    • Author(s)
      Y. Kato, T. Noguchi
    • Journal Title

      Biochemistry

      Volume: 53 Issue: 30 Pages: 4914-4923

    • DOI

      10.1021/bi500549b

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 光合成光化学系IIにおけるMnクラスターと第一キノン電子受容体QAの長距離相互作用2016

    • Author(s)
      加藤 祐樹, 石井 里奈, 野口 巧
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学 京田辺キャンパス(京都府京田辺市)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] ATR-FTIR analysis of the long-range interaction between the Mn cluster and the primary quinone QA in photosystem II2016

    • Author(s)
      加藤 祐樹, 石井 里奈, 野口 巧
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学(岩手県盛岡市)
    • Year and Date
      2016-03-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 光化学系IIにおけるキノンQA・QB間の電子伝達制御機構の解明:QBの酸化還元電位計測2016

    • Author(s)
      加藤 祐樹, 長尾 遼, 野口 巧
    • Organizer
      平成27年度生物物理学会中部支部講演会
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県岡崎市)
    • Year and Date
      2016-02-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] Redox potential of the secondary quinone electron acceptor QB in photosystem II as revealed by FTIR spectroelectrochemistry2015

    • Author(s)
      Yuki Kato, Ryo Nagao, Takumi Noguchi
    • Organizer
      International Meeting Photosynthesis Research for Sustainability -2015
    • Place of Presentation
      Orthodox Academy of Crete, Chania, Crete, Greece
    • Year and Date
      2015-09-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] FTIR spectroelectrochemical measurement of the redox potential of the secondary quinone electron acceptor QB in photosystem II2015

    • Author(s)
      Yuki Kato, Ryo Nagao, Takumi Noguchi
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学 角間キャンパス(石川県金沢市)
    • Year and Date
      2015-09-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] 光合成光化学系II複合体における第二キノン電子受容体QBの酸化還元電位測定2015

    • Author(s)
      加藤 祐樹, 長尾 遼, 野口 巧
    • Organizer
      2015年電気化学秋季大会
    • Place of Presentation
      埼玉工業大学(埼玉県)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] 光化学系II第二キノン QBの二電子還元反応の解析2015

    • Author(s)
      加藤 祐樹, 長尾 遼, 野口 巧
    • Organizer
      第23回光合成セミナー2015
    • Place of Presentation
      龍谷大学 大宮キャンパス(京都市)
    • Year and Date
      2015-07-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] FTIR Spectroelectrochemical Study on Long-Range Interaction between the Mn4CaO5 Cluster and the Non-Heme Iron Center in Photosystem II2015

    • Author(s)
      Yuki Kato, Takumi Noguchi
    • Organizer
      "Metals in Biology" in Wako
    • Place of Presentation
      RIKEN Wako Campus(埼玉県和光市)
    • Year and Date
      2015-06-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 光合成光化学系IIにおけるキノン電子受容体QA・QB間の電子伝達制御: QBの酸化還元電位計測に基づく解析2015

    • Author(s)
      加藤 祐樹, 長尾 遼, 野口 巧
    • Organizer
      第42回生体分子科学討論会
    • Place of Presentation
      高崎シティーギャラリー(群馬県高崎市)
    • Year and Date
      2015-06-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] Mnクラスターの損傷に対する光化学系IIキノンQA・QB間の電子伝達制御と光防御機構 −QBの酸化還元電位計測による解析2015

    • Author(s)
      加藤 祐樹, 長尾 遼, 野口 巧
    • Organizer
      第6回日本光合成学会年会
    • Place of Presentation
      岡山国際交流センター(岡山県岡山市)
    • Year and Date
      2015-05-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 光合成光化学系II第二キノンQBの酸化還元電位測定 -FTIR分光電気化学法によるアプローチ2015

    • Author(s)
      加藤 祐樹, 長尾 遼, 野口 巧
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      日本大学 理工学部船橋キャンパス(千葉県・船橋市)
    • Year and Date
      2015-03-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 光合成における光化学系II第二キノン電子受容体QBの酸化還元電位計測2015

    • Author(s)
      加藤 祐樹, 長尾 遼, 野口 巧
    • Organizer
      日本物理学会第70回年次大会
    • Place of Presentation
      早稲田大学(東京都)
    • Year and Date
      2015-03-21
    • Related Report
      2014 Research-status Report
  • [Presentation] 光化学系IIにおける第二キノン電子受容体QBの首振り機構による電子・プロトン伝達2015

    • Author(s)
      嘉手川千央、加藤祐樹、野口巧
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学(東京都)
    • Year and Date
      2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] FTIR分光電気化学法による光化学系II第二キノンQBの酸化還元電位計測 −QA・QB間の電位制御と光防御機構2015

    • Author(s)
      加藤 祐樹, 長尾 遼, 野口 巧
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学(東京都)
    • Year and Date
      2015-03-17
    • Related Report
      2014 Research-status Report
  • [Presentation] 光合成光化学系IIにおけるMnクラスターの損傷が電子受容側機能分子の酸化還元電位に及ぼす影響2014

    • Author(s)
      加藤祐樹, 野口 巧
    • Organizer
      2014年電気化学秋季大会
    • Place of Presentation
      北海道大学(北海道・札幌市)
    • Year and Date
      2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Electron and proton transfer mechanism of the secondary quinone electron acceptor QB in photosystem II2014

    • Author(s)
      Yukihiro Kadekawa, Yuki Kato, Takumi Noguchi
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌市)
    • Year and Date
      2014-09-25
    • Related Report
      2014 Research-status Report
  • [Presentation] FTIR spectroelectrochemical study on the influence of Mn-depletion on the redox potential of the non-heme iron and its surrounding structure in photosystem II2014

    • Author(s)
      Yuki Kato, Takumi Noguchi
    • Organizer
      International Conference "Photosynthesis Research for Sustainability"
    • Place of Presentation
      RAS, Puschino, Russia
    • Year and Date
      2014-06-04
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 光化学系IIにおけるMnクラスターの損傷がアクセプター側機能分子に及ぼす影響 -FTIR分光電気化学計測による解析2014

    • Author(s)
      加藤 祐樹, 野口 巧
    • Organizer
      平成25年度生物物理学会中部支部会講演会
    • Place of Presentation
      岡崎コンファレンスセンター
    • Related Report
      2013 Research-status Report
  • [Presentation] 光化学系IIにおいてMn除去は非ヘム鉄の酸化還元電位を変動させるか?2014

    • Author(s)
      加藤 祐樹, 野口 巧
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 光合成光化学系IIにおけるMnクラスターの損傷が非ヘム鉄の酸化還元電位に及ぼす影響2014

    • Author(s)
      加藤 祐樹, 野口 巧
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 光化学系IIにおけるMn除去が非ヘム鉄の酸化還元電位に及ぼす影響 -FTIR-分光電気化学法によるアプローチ2013

    • Author(s)
      加藤祐樹, 野口 巧
    • Organizer
      第4回日本光合成学会年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Mn除去による光化学系II非ヘム鉄の酸化還元電位の変動2013

    • Author(s)
      加藤祐樹, 野口 巧
    • Organizer
      第21回光合成セミナー
    • Place of Presentation
      名古屋工業大学
    • Related Report
      2013 Research-status Report
  • [Presentation] nfluence of the Mn-Depletion on the Redox Potential of the Non-Heme Iron in Photosystem II as revealed by FTIR-Spectroelectrochemistry2013

    • Author(s)
      Yuki Kato, Takumi Noguchi
    • Organizer
      The 16th International Congress on Photosynthesis
    • Place of Presentation
      St. Louis Hyatt Regency at the Arch
    • Related Report
      2013 Research-status Report
  • [Presentation] FTIR-Spectroelectrochemical Study on the Influence of the Mn-Depletion from Photosystem II on the Redox Potential of the Non-Heme Iron2013

    • Author(s)
      Yuki Kato, Takumi Noguchi
    • Organizer
      Seventh International Conference on Advanced Vibrational Spectroscopy -7th ICAVS
    • Place of Presentation
      神戸コンベンションセンター
    • Related Report
      2013 Research-status Report
  • [Presentation] FTIR-Spectroelectrochemical Investigation into Whether the Mn-Depletion influences the Redox Potential of the Non-Heme Iron in Photosystem II2013

    • Author(s)
      Yuki Kato, Takumi Noguchi
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Research-status Report
  • [Book] 光合成のエネルギー変換と物質変換2015

    • Author(s)
      加藤祐樹(共著)
    • Total Pages
      281
    • Publisher
      化学同人
    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi