| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
Aiming at a practical protein structure analysis with fluorescence-detected CD, structure-spectrum relationship was investigated by using a synthetic library of oligopeptides that contain Trp as a fluorescent amino acid. Trp was derivatized systematically with a variety of oligoprolines as rigid spacers. As a result of careful comparison among those FDCD spectra, it was inferred from the expansion at Trp C-terminal that observable are two interactions, the coupling of Trp with neighboring peptides and the energy transfer from Phe to Trp over the spacer. On the other hand, the expansion at Trp N-terminal implied that the coupling with neighboring peptides would be suppressed. A micro-scale cell that requires only a tenth of volumes was also developed.
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