| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Outline of Final Research Achievements |
CIEEL active dioxetanes bearing an aromatic electron donor display chemiluminescence by CTID mechanism, in which CT occurs from the electron donor to dioxetane O-O to produce excited species. We have recently found that CTID occurred for dioxetanes bearing a hydroxyphenyl moiety substituted further with an aryl that acted as an antenna to capture an electron from a matrix in negative-mode MALDI-TOF-Mass. In this work, we found that a bicyclic dioxetane bearing a phenol with such antenna captured an electron from matrix to decompose a keto ester, in which the ketone moiety but not the aromatic ester was in the an excited state. On the other hand, a vibrationally excited keto ester formed by collision induced dissociation in MS/MS.
In the coure of such investigation of dioxetanes we found that a quinacridone derivative was useful as a new matrix under negative-mode. When a planar organic base such as phenazine is added to matrix the ionization efficiency is markedly improved.
|
