Analysis of elementary process for chemi-excitation of CIEEL type dioxetanes by the use of photo-induced ionization in MALDI
| Project/Area Number |
25410057
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Organic chemistry
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| Research Institution | Kanagawa University |
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Principal Investigator |
Ijuin Hisako 神奈川大学, 付置研究所, 研究員 (60398948)
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| Co-Investigator(Kenkyū-buntansha) |
Masakatsu Matsumoto 神奈川大学, 光機能性材料研究所, プロジェクト研究員 (10260986)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 化学発光 / MALDI法質量分析 / ジオキセタン / ラジカルイオン種 / 電子移動 |
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| Outline of Final Research Achievements |
CIEEL active dioxetanes bearing an aromatic electron donor display chemiluminescence by CTID mechanism, in which CT occurs from the electron donor to dioxetane O-O to produce excited species. We have recently found that CTID occurred for dioxetanes bearing a hydroxyphenyl moiety substituted further with an aryl that acted as an antenna to capture an electron from a matrix in negative-mode MALDI-TOF-Mass. In this work, we found that a bicyclic dioxetane bearing a phenol with such antenna captured an electron from matrix to decompose a keto ester, in which the ketone moiety but not the aromatic ester was in the an excited state. On the other hand, a vibrationally excited keto ester formed by collision induced dissociation in MS/MS.
In the coure of such investigation of dioxetanes we found that a quinacridone derivative was useful as a new matrix under negative-mode. When a planar organic base such as phenazine is added to matrix the ionization efficiency is markedly improved.
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Report
(4 results)
Research Products
(6 results)
