Regulation of photochemical reaction by grapheme oxide and its application to gene analysis
| Project/Area Number |
25410146
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | DNA / グラフェン / バイオセンサー / 遺伝子解析 / 光化学反応 / 酸化グラフェン |
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| Outline of Final Research Achievements |
Partially oxidized graphene (graphene oxide, GO) is an atomically thin, two-dimensional sheet that contains nanometer-scale graphene-like sp2 domains in an amorphous sp3 carbon matrix. It can strongly adsorb single-stranded DNA (ssDNA) via non-covalent hydrophobic and π-stacking interactions with the exposed nucleobases. Meanwhile, GO affinity for double-stranded DNA is much lower because the nucleobases are shielded inside the hydrophilic double helix backbone. GO is also a very effective fluorescence quencher owing to its efficient, long-range energy transfer properties.
In this research, fluorescent-dye-labeled probe DNA was immobilized on fluorescence-quenching graphene oxide (GO) through a capture DNA. When targets were added, the probes were released from the GO through toehold-mediated strand exchange. Higher emission recovery and more signal contrast were achieved relative to conventional methods that are based on direct adsorption of probes.
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Report
(4 results)
Research Products
(35 results)
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[Journal Article] In Situ Oxygenous Functionalization of a Graphite Electrode for Enhanced Affinity toward Charged Species and a Reduced Graphene Oxide Mediator2014
Author(s)
M. R. Karim, Y. Ikeda, T. Ide, S. Sugito, K. Toda, YM. R. Karim, Y. Ikeda, T. Ide, S. Sugimoto, K. Toda, Y. Kitamura, T. Ihara, T. Matsui, T. Tani. Kitamura, T. Ihara, T. Matsui, T. Taniguchi, M. Koinuma, Y. Matsumoto, S. Hayami
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Journal Title
New Journal of Chemistry
Volume: 38
Issue: 5
Pages: 2120-2127
DOI
Related Report
Peer Reviewed / Open Access
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