| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
|
|---|
| Outline of Final Research Achievements |
Partially oxidized graphene (graphene oxide, GO) is an atomically thin, two-dimensional sheet that contains nanometer-scale graphene-like sp2 domains in an amorphous sp3 carbon matrix. It can strongly adsorb single-stranded DNA (ssDNA) via non-covalent hydrophobic and π-stacking interactions with the exposed nucleobases. Meanwhile, GO affinity for double-stranded DNA is much lower because the nucleobases are shielded inside the hydrophilic double helix backbone. GO is also a very effective fluorescence quencher owing to its efficient, long-range energy transfer properties.
In this research, fluorescent-dye-labeled probe DNA was immobilized on fluorescence-quenching graphene oxide (GO) through a capture DNA. When targets were added, the probes were released from the GO through toehold-mediated strand exchange. Higher emission recovery and more signal contrast were achieved relative to conventional methods that are based on direct adsorption of probes.
|
