Development of functional biochips with nano-scale layered structure for sensitive proteomics

• Project/Area Number: 25410151
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Tokyo University of Technology
• Principal Investigator: YANO Kazuyoshi 東京工科大学, 応用生物学部, 教授 (40262109)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: プロテオミクス / プラズマ重合 / 分析化学 / イムノアッセイ / 蛍光 / 薄膜

Outline of Final Research Achievements

Nano-scale layered structure was fabricated on a 96-well microplate modified with silver layer as a metal mirror and with plasma-polymerized film as an optical interference layer. Fluorescence-based sandwich immunoassay targeting C-reactive protein and interleukin-2 was performed using the microplate. It was shown that enhanced fluorescence signal was obtained with the modified microplate compared with the unmodified one, resulting in highly sensitive detection.

Research Products

• [Journal Article] Fluorescence Enhancement of Immunoassay Using Multilayered Glass Substrates Modified with Plasma-Polymerized Films (2015)
  • Author(s): Kazuyoshi Yano, Kazuyuki Yamano, Akira Iwasaki, Takuo Akimoto, Hirotaka Miyachi1 and Atsunori Hiratsuka
  • Journal Title: Sensors and Materials Volume: 27 Pages: 859-869
  • DOI: 10.18494/SAM.2015.1122
  • ISSN: 0914-4935, 2435-0869
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Characterization of the DNA aptamer which binds to CD4 and its application for detection of CD4-expressing cells (2015)
  • Author(s): Kazuyoshi Yano, Toshinao Shibata, Yui Sato, Hitomi Kubo, Tomoo Shimizu, Akiko Shiratani, Masafumi Shimizu, Atsushi Sato
  • Organizer: 2015 International Chemical Congress of Pacific Basin Societies (PACIFICHEM2015)
  • Place of Presentation: Hawaii Convention Center (Honolulu, Hawaii, USA)
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] CD4に結合するDNAアプタマーの解析とCD4発現細胞の検出への応用 (2015)
  • Author(s): 矢野 和義、柴田 俊奈生、西山 銀侍、清水 智夫、白谷 明子、清水 雅史、片岡 尚希、佐藤 淳
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸国際展示場（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] CD4に結合するDNAアプタマーの解析とCD4発現細胞の検出への応用
  • Author(s): 柴田俊奈生、清水智夫、白谷明子、清水雅史、片岡尚希、佐藤淳、矢野和義
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋大学東山キャンパス（名古屋市千種区不老町）
• [Presentation] Enhancement of binding affinity and structural characterization of the anti-CD4 aptamer
  • Author(s): 矢野和義、佐藤唯、久保仁美、清水智夫、白谷明子、清水雅史
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場（神戸市中央区港島中町）