Development of Biomedical Analysis Based on Monodisperse Microdroplet
Project/Area Number |
25410153
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Analytical chemistry
|
Research Institution | Doshisha University |
Principal Investigator |
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 単分散油中水滴の自動調製 / 単一DNA分子の検出 / 変異DNAの絶対的定量 / エマルジョン / 油中水滴 / デジタルPCR / ポリジメチルシロキサン / マイクロ流体デバイス / 単一分子 / 変異検出 / DNA / 単分散性 / 一分子PCR / マイクロ流体チップ |
Outline of Final Research Achievements |
A fully autonomous method of preparing highly monodispersed water-in-oil droplets with a low sample dead volume was realized using an air-evacuated poly(dimethylsiloxane) (PDMS) microfluidic chip possessing a simple T-junction channel geometry with two inlet reservoirs for oil and water to be loaded and one outlet reservoir for the collection of generated droplets. Autonomous transport of oil and water phases in the channel was realized by permeation of air confined inside the outlet reservoir into the degassed PDMS. The only operation required for droplet preparation was simple pipetting of oil and aqueous solutions into the inlet reservoirs. It was verified that the resultant droplets could serve as microreactors for digital polymerase chain reactions.
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Report
(4 results)
Research Products
(24 results)