Construction of double-strand RNA in cell by cross-link reaction
| Project/Area Number |
25410165
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
SATO Kousuke 北海道大学, 薬学研究科(研究院), 助教 (70415686)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 核酸化学 / 核酸医薬品 / ヌクレオシド誘導体 / クロスリンク反応 / オリゴヌクレオチド |
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| Outline of Final Research Achievements |
We synthesized a C-nucleoside containing thiol group for cross-link reactions, and incorporated into oligodeoxynucleotides by use of phosphoramidite units.
4-Halopyridine-C-nucleosides were also synthesized for cross-link reaction with thiol group. These C-nucleosides were highly reactive with mercaptoethanol. Therefore, these C-nucleosides applied to nucleic acid drugs by covalent bond formation with thiol group in target protein. Modified oligonucleotides formed covalent complex with target protein, and inhibited the cancer cell growth.
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Report
(4 results)
Research Products
(22 results)
