| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
We synthesized a C-nucleoside containing thiol group for cross-link reactions, and incorporated into oligodeoxynucleotides by use of phosphoramidite units.
4-Halopyridine-C-nucleosides were also synthesized for cross-link reaction with thiol group. These C-nucleosides were highly reactive with mercaptoethanol. Therefore, these C-nucleosides applied to nucleic acid drugs by covalent bond formation with thiol group in target protein. Modified oligonucleotides formed covalent complex with target protein, and inhibited the cancer cell growth.
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