Development of a photoresponsive module for protein photoregulation
| Project/Area Number |
25410166
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
Ui Mihoko 東北大学, 多元物質科学研究所, 助教 (30549580)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 光制御 / 光受容蛋白質 / PYP / 光応答性材料 / クリック反応 / 非天然アミノ酸導入 / モジュール化 |
|---|
| Outline of Final Research Achievements |
Light has been considered as an effective tool to regulate biomolecules due to its high spatiotemporal resolution and noninvasive properties. In this study, we developed photoresponsive modules based on photoreceptor PYP by providing an unnatural amino acid bearing a chemically reactive group instead of methionine. The engineered PYP was coupled with the hydrophilic copolymer, resulting in generation of photoresponsive protein-polymer conjugates. The viscosity of the resulting conjugate was reversibly decreased by blue light irradiation. This material would possibly be applicable for construction of photoresponsive biomaterials and also broaden the potential of engineered PYP as a photoresponsive module for optical regulation of molecules.
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Report
(4 results)
Research Products
(26 results)
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[Presentation] Engineering of Mono-Disperse Oligoethylene Glycols for Protein Manipulation2014
Author(s)
K. Kinbara, T. Muraoka, K. Adachi, M. Ui, S. Kawasaki, N. Sadhukhan, H. Obara, M. Laguerre, H. Tochio, M. Shirakawa
Organizer
ASIAN CHEMICAL BIOLOGY COFERENCE 3(ACBC2014)
Place of Presentation
シンガポール共和国,シンガポール
Year and Date
2014-12-15 – 2014-12-17
Related Report
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[Presentation] 機能性分子導入型タンパク質の構築2014
Author(s)
丸山翔太, 宇井美穂子, 三浦紗理, 村岡貴博, 高橋泰人, 金原数
Organizer
第4回CSJ化学フェスタ2014 ―日本化学会秋季事業―
Place of Presentation
東京都
Year and Date
2014-10-14 – 2014-10-16
Related Report
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[Presentation] 光応答性タンパク質を用いた新規機能性材料の創製2014
Author(s)
前島 辰哉 , 宇井 美穂子 , 宮内 佑輔 , 村上 慎 , 荒木 保幸 , 和田 健彦 , 金原 数
Organizer
第4回CSJ化学フェスタ2014 ―日本化学会秋季事業―
Place of Presentation
東京都
Year and Date
2014-10-14 – 2014-10-16
Related Report
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