• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of a photoresponsive module for protein photoregulation

Research Project

Project/Area Number 25410166
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bio-related chemistry
Research InstitutionTohoku University

Principal Investigator

Ui Mihoko  東北大学, 多元物質科学研究所, 助教 (30549580)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords光制御 / 光受容蛋白質 / PYP / 光応答性材料 / クリック反応 / 非天然アミノ酸導入 / モジュール化
Outline of Final Research Achievements

Light has been considered as an effective tool to regulate biomolecules due to its high spatiotemporal resolution and noninvasive properties. In this study, we developed photoresponsive modules based on photoreceptor PYP by providing an unnatural amino acid bearing a chemically reactive group instead of methionine. The engineered PYP was coupled with the hydrophilic copolymer, resulting in generation of photoresponsive protein-polymer conjugates. The viscosity of the resulting conjugate was reversibly decreased by blue light irradiation. This material would possibly be applicable for construction of photoresponsive biomaterials and also broaden the potential of engineered PYP as a photoresponsive module for optical regulation of molecules.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (26 results)

All 2016 2015 2014 2013 2012 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Acknowledgement Compliant: 2 results) Presentation (20 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Protein stabilization by an amphiphilic short monodisperse oligo(ethylene glycol)2015

    • Author(s)
      Nabanita Sadhukhan, Takahiro Muraoka, Mihoko Ui, Satoru Nagatoishi, Kouhei Tsumoto, Kazushi Kinbara
    • Journal Title

      Chemical Communications

      Volume: 51 Issue: 40 Pages: 8457-8460

    • DOI

      10.1039/c4cc10301g

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Protein stabilization by an amphiphilic short monodisperse oligo(ethylene glycol).2015

    • Author(s)
      Sadhukhan N, Muraoka T, Ui M, Nagatoishi S, Tsumoto K, Kinbara K.
    • Journal Title

      Chemical communications : Chem comm / the Royal Society of Chemistry.

      Volume: in press

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Grafting Synthetic Transmembrane Units to the Engineered Low-Toxicity alpha-Hemolysin to Restore Its Hemolytic Activity2014

    • Author(s)
      Mihoko Ui, Kousuke Harima, Toshiaki Takei, Kouhei Tsumoto, Kazuhito V. Tabata, Hiroyuki Noji, Sumire Endo, Kimio Akiyama, Takahiro Muraoka, and Kazushi Kinbara
    • Journal Title

      Molecular Biosystems

      Volume: 10 Issue: 12 Pages: 3199-3206

    • DOI

      10.1039/c4mb00405a

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Thermal-Aggregation Suppression of Proteins by a Structured PEG Analogue : Importance of Denaturation Temperature for Effective Aggregation Suppression2014

    • Author(s)
      Takahiro Muraoka, Nabanita Sadhukhan, Mihoko Ui, Shunichi Kawasaki, Enrikko Hazemi, Kota Adachi, and Kazushi Kinbara
    • Journal Title

      Biochem. Eng. J

      Volume: 86C Pages: 41-48

    • DOI

      10.1016/j.bej.2014.03.001

    • Related Report
      2014 Research-status Report 2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A Structured Monodisperse PEG for the Effective Suppression of Protein Aggregation2013

    • Author(s)
      Takahiro Muraoka, Kota Adachi, Mihoko Ui, Shunichi Kawasaki, Nabanita Sadhukhan, Hamki Obara, Hidehito Tochio, Masahiro Shirakawa, and Kazushi Kinbara
    • Journal Title

      Angew. Chem., Int. Ed

      Volume: 52 Issue: 9 Pages: 2430-2434

    • DOI

      10.1002/anie.201206563

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mutations for decreasing the immunogenicity and maintaining the function of core streptavidin.2012

    • Author(s)
      Yumura K
    • Journal Title

      Protein Sci.

      Volume: Feb;22(2) Issue: 2 Pages: 213-221

    • DOI

      10.1002/pro.2203

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Development of functional material using an engineered reactive photoreceptor2016

    • Author(s)
      MAEJIMA, Tatsuya; UI, Mihoko; ARAKI, Yasuyuki; WADA, Takehiko; KINBARA, Kazushi
    • Organizer
      日本化学会 第96春季年会
    • Place of Presentation
      同志社大学
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] Application of photoactive yellow protein as a photoresponsive module for controlling enzymatic activity2015

    • Author(s)
      Mihoko Ui, Yasuhiro Arai, Makoto Murakami, Yasuyuki Araki, Takehiko Wada, Hiroto Takahashi, Kazushi Kinbara
    • Organizer
      THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES 2015
    • Place of Presentation
      アメリカ合衆国,ハワイ州ホノルル
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of Photoresponsive Functional Material Utilizing the Engineered Photoactive Yellow Protein2015

    • Author(s)
      Tatsuya Maejima, Mihoko Ui, Yasuyuki Araki, Kazushi Kinbara, Takehiko Wada
    • Organizer
      Chemistry Summer School 2015
    • Place of Presentation
      東北大学
    • Year and Date
      2015-08-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Construction of PEG-incorporated Synthetic Protein2015

    • Author(s)
      Shouta Maruyama, Mihoko Ui, Takahiro Muraoka, Kazushi Kinbara
    • Organizer
      Chemistry Summer School 2015
    • Place of Presentation
      東北大学
    • Year and Date
      2015-08-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] PEG導入型人工タンパク質の構築2015

    • Author(s)
      丸山翔太、宇井美穂子、村岡貴博、高橋泰人、 金原数
    • Organizer
      日本化学会第95春季年会
    • Place of Presentation
      船橋市
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 光応答性タンパク質の特性を利用した機能性材料の創製2015

    • Author(s)
      前島辰哉、宇井美穂子、荒木保幸、和田健彦、金原数
    • Organizer
      日本化学会第95春季年会
    • Place of Presentation
      船橋市
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] Engineering of Mono-Disperse Oligoethylene Glycols for Protein Manipulation2014

    • Author(s)
      K. Kinbara, T. Muraoka, K. Adachi, M. Ui, S. Kawasaki, N. Sadhukhan, H. Obara, M. Laguerre, H. Tochio, M. Shirakawa
    • Organizer
      ASIAN CHEMICAL BIOLOGY COFERENCE 3(ACBC2014)
    • Place of Presentation
      シンガポール共和国,シンガポール
    • Year and Date
      2014-12-15 – 2014-12-17
    • Related Report
      2014 Research-status Report
  • [Presentation] 機能性分子導入型タンパク質の構築2014

    • Author(s)
      丸山翔太, 宇井美穂子, 三浦紗理, 村岡貴博, 高橋泰人, 金原数
    • Organizer
      第4回CSJ化学フェスタ2014 ―日本化学会秋季事業―
    • Place of Presentation
      東京都
    • Year and Date
      2014-10-14 – 2014-10-16
    • Related Report
      2014 Research-status Report
  • [Presentation] 光応答性タンパク質を用いた新規機能性材料の創製2014

    • Author(s)
      前島 辰哉 , 宇井 美穂子 , 宮内 佑輔 , 村上 慎 , 荒木 保幸 , 和田 健彦 , 金原 数
    • Organizer
      第4回CSJ化学フェスタ2014 ―日本化学会秋季事業―
    • Place of Presentation
      東京都
    • Year and Date
      2014-10-14 – 2014-10-16
    • Related Report
      2014 Research-status Report
  • [Presentation] 生体機能制御を指向した光応答性タンパク質の構築と細胞性粘菌を用いた評価2014

    • Author(s)
      宇井美穂子, 新井康広, 村上慎, 荒木保幸, 和田健彦, 高橋泰人, 金原数
    • Organizer
      第4回 日本細胞性粘菌学会 年会
    • Place of Presentation
      仙台市
    • Year and Date
      2014-10-11 – 2014-10-12
    • Related Report
      2014 Research-status Report
  • [Presentation] 光応答性PYP融合酵素を用いたcAMP濃度制御システムの構築2014

    • Author(s)
      宇井美穂子, 新井康広, ,村上 慎, 荒木保幸, 和田健彦, 高橋泰人, 金原 数
    • Organizer
      平成25年度アライアンス成果報告会
    • Place of Presentation
      豊中市
    • Year and Date
      2014-05-30
    • Related Report
      2014 Research-status Report
  • [Presentation] Stabilization of proteins by a structured oligoethlene glycol analogues with non-linear geometry2014

    • Author(s)
      Kazushi Kinbara, Takahiro Muraoka, Kota Adachi, Mihoko Ui, Shunichi Kawasaki, Nabanita Sadhukhan, Haruki Obara
    • Organizer
      10th International Conference on. Protein Stabilisation (ProtStab2014)
    • Place of Presentation
      イタリア、ストレーザ
    • Year and Date
      2014-05-07 – 2014-05-09
    • Related Report
      2014 Research-status Report
  • [Presentation] The amphiphilic PEG additives for suppression of protein aggregation2014

    • Author(s)
      Mihoko Ui, Nabanita Sadhukhan, Takahiro Muraoka, Kazushi Kinbara
    • Organizer
      10th International Conference on. Protein Stabilisation
    • Place of Presentation
      イタリア、ストレーザ
    • Year and Date
      2014-05-07 – 2014-05-09
    • Related Report
      2014 Research-status Report
  • [Presentation] Constructing nano-pore devices based on pore-forming toxin α-hemolysin by replacing its transmembrane region with chemical compounds2013

    • Author(s)
      Mihoko UI, Kousuke HARIMA, Yoshikazu TANAKA, Kazuhito TABATA,  Hiroyuki Noji, Toshiaki TAKEI, Kouhei TSUMOTO, Kazushi KINBARA
    • Organizer
      NIH-Tohoku University-JSPS国際シンポジウム
    • Place of Presentation
      東北大学艮陵会館
    • Related Report
      2013 Research-status Report
  • [Presentation] Creation of a Photoresponsive module based on the engineered PYP2013

    • Author(s)
      Yusuke Miyauchi, Mihoko Ui, Makoto Murakami, Yasuyuki Araki, Takehiko Wada, and Kazushi Kinbara
    • Organizer
      Tohoku University Campus Asia Summer School 2013
    • Place of Presentation
      東北大学大学青葉山キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] A photocontrollable nanopore-device based on chemically Hybridized pore-forming toxin alpha-hemolysin2013

    • Author(s)
      M. Ui, K. Harima, Y. Tanaka, K. Tabata, H. Noji, T. Takei, K. Tsumoto and K. Kinbara
    • Organizer
      Faraday Discussion 166: Self Assembly of Biopolymers
    • Place of Presentation
      University of Bristol、イギリス、ブリストル
    • Related Report
      2013 Research-status Report
  • [Presentation] 光応答性蛋白質を用いたcAMP濃度制御システムの構築2013

    • Author(s)
      宇井 美穂子、新井 康広、村上 慎、荒木 保幸、和田 健彦、高橋 泰人、金原 数
    • Organizer
      第7回バイオ関連化学シンポジウム
    • Place of Presentation
      名古屋大学東山キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] 光応答性バイオマテリアルへの応用を指向したモジュール化PYPの創製2013

    • Author(s)
      宮内 佑輔、宇井 美穂子、村上 慎、荒木 保幸、和田 健彦、金原 数
    • Organizer
      第7回バイオ関連化学シンポジウム
    • Place of Presentation
      名古屋大学東山キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] Construction of a photocontrol system of cAMP level based on the photoresponsive phosphodiesterase2013

    • Author(s)
      Mihoko Ui, Yasuhiro Arai, Makoto Murakami, Yasuyuki Araki, Takehiko Wada, Hiroto Takahashi, and Kazushi Kinbara
    • Organizer
      平成25年度化学系学協会東北大会及び日本化学会東北支部70周年記念国際会議 ―International Symposium for the 70th Anniversary of the Tohoku Branch Chemical Society of Japan―
    • Place of Presentation
      東北大学川内北キャンパス・萩ホール
    • Related Report
      2013 Research-status Report
  • [Presentation] 光受容蛋白質を用いた細胞外cAMP濃度制御システムの構築

    • Author(s)
      宇井美穂子、新井康広、村上 慎、荒木保幸、和田健彦、高橋泰人、金原 数
    • Organizer
      第13回東北大学多元物質科学研究所 研究発表会
    • Place of Presentation
      東北大学片平キャンパス
    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi