| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
Light has been considered as an effective tool to regulate biomolecules due to its high spatiotemporal resolution and noninvasive properties. In this study, we developed photoresponsive modules based on photoreceptor PYP by providing an unnatural amino acid bearing a chemically reactive group instead of methionine. The engineered PYP was coupled with the hydrophilic copolymer, resulting in generation of photoresponsive protein-polymer conjugates. The viscosity of the resulting conjugate was reversibly decreased by blue light irradiation. This material would possibly be applicable for construction of photoresponsive biomaterials and also broaden the potential of engineered PYP as a photoresponsive module for optical regulation of molecules.
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