• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Sensitivity improvement of Elliptically-polarization-detected CD spectroscopy for the drug-biomolecule binidng study

Research Project

Project/Area Number 25410167
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bio-related chemistry
Research InstitutionTohoku University

Principal Investigator

ARAKI Yasuyuki  東北大学, 多元物質科学研究所, 准教授 (80361179)

Co-Investigator(Renkei-kenkyūsha) WADA Takehiko  東北大学, 多元物質科学研究所, 教授 (20220957)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsキラリティ / 円二色性 / 高感度検出 / 微弱光検出 / フォトンカウンティング
Outline of Final Research Achievements

Cationic porphyrins are well-known as small molecules bound to double strand DNA (dsDNA) and also known as typical reagents of the photodynamic therapy.
Recently, we reported new methodology for high sensitive circular dichroism (CD) spectroscopy and application for the analysis of the interaction dynamics of cationic porphyrins with dsDNA.
In this study, we demonstrated the variety of cationic porphyrin derivatives with dsDNAs formed the intercalating complexes. Our time resolved CD measurement system revealed that the photo-irradiation of porphyrins intercalated to dsDNA showed the photo-bleaching, those were the originated from the photo-excited triplet state generating inside dsDNA. Transient absorption study for these porphyrin-dsDNA complexes were also consistent with these transient CD data. Thus we have concluded that we have successfully observed the photo-dynamics of porphyrin-dsDNA complexes by using our transient CD technique.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (21 results)

All 2016 2015 2014 2013

All Journal Article (11 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 11 results,  Open Access: 1 results,  Acknowledgement Compliant: 5 results) Presentation (10 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Synthetic Control of Photophysical Process and Circularly Polarized Luminescence of [5]Carbohelicene Derivatives Substituted by Maleimide Units2016

    • Author(s)
      Hayato Sakai, Takako Kubota, Junpei Yuasa, Yasuyuki Araki, Tomo Sakanoue, Taishi Takenobu, Takehiko Wada, Tsuyoshi Kawai, and Taku Hasobe
    • Journal Title

      Journal of Phyisical Chemistry C

      Volume: 120 Issue: 14 Pages: 7860-7869

    • DOI

      10.1021/acs.jpcc.6b01344

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Controlled Excited-State Dynamics and Enhanced Fluorescence Property of Tetrasulfone[9]helicene by a Simple Synthetic Process2016

    • Author(s)
      Yamamoto, Y; Sakai, H ; Yuasa, J ; Araki, Y; Wada, T ; Sakanoue, T ; Takenobu, T ; Kawai, T; Hasobe, T
    • Journal Title

      JOURNAL OF PHYSICAL CHEMISTRY C

      Volume: 120 Issue: 13 Pages: 7421-7427

    • DOI

      10.1021/acs.jpcc.6b01123

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Long-Lived Triplet Excited States of Bent-Shaped Pentacene Dimers by Intramolecular Singlet Fission2016

    • Author(s)
      T. Sakuma, H. Sakai, Y. Araki, T. Mori, T. Wada, N. V. Tkachenko, T. Hasobe
    • Journal Title

      J. Phys. Chem. A

      Volume: 120 Issue: 11 Pages: 1867-1875

    • DOI

      10.1021/acs.jpca.6b00988

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Synthetic Control of the Excited-State Dynamics and Circularly Polarized Luminescence of Fluorescent "push-Pull" Tetrathia[9]helicenes2016

    • Author(s)
      Yuki Yamamoto, Hayato Sakai, Junpei Yuasa, Yasuyuki Araki, Takehiko Wada, Tomo Sakanoue, Taishi Takenobu, Tsuyoshi Kawai, Taku Hasobe
    • Journal Title

      Chemistry - A European Journal

      Volume: 22 Issue: 12 Pages: 4263-4273

    • DOI

      10.1002/chem.201504048

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Highly Fluorescent [7]Carbohelicene Fused by Asymmetric 1,2-Dialkyl-Substituted Quinoxaline for Circularly Porlarized Luminescence and Electroluminescence2015

    • Author(s)
      H. Sakai, S. Shinto, J. Kumar, Y. Araki, T. Sakanoue, T. Takenobu, T. Wada, T. Kawai, T. Hasobe
    • Journal Title

      J. Phys. Chem. C

      Volume: 119 Issue: 24 Pages: 13937-13947

    • DOI

      10.1021/acs.jpcc.5b03386

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 生体機能分子の構造変化検出を指向した時間分解円二色性測定法の開発2015

    • Author(s)
      荒木保幸、和田健彦
    • Journal Title

      光化学

      Volume: 46 Pages: 41-44

    • NAID

      40020462347

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Formation of One-Dimensional Helical Columns and Excimerlike Excited States by Racemic Quinoxaline-Fused [7] Carbohelicenes in the Crystal,2014

    • Author(s)
      H. Sakai, S. Shinto, Y. Araki, T. Wada, T. Sakanoue, T. Takenobu, T. Hasobe
    • Journal Title

      Chem. - A Eur. J

      Volume: 20 Issue: 32 Pages: 10099-10109

    • DOI

      10.1002/chem.201402426

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Systematic control of the excited-state dynamics and carrier-transport properties of functionalized benzo[ghi]perylene and coronene derivatives2014

    • Author(s)
      S. Hirayama, H. Sakai, Y. Araki, M. Tanaka, M. Imakawa, T. Wada, T. Takenobu, T. Hasobe
    • Journal Title

      Chem.- A Eur. J

      Volume: 20 Issue: 29 Pages: 9081-9093

    • DOI

      10.1002/chem.201304679

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Electron-Transfer Reduction Properties and Excited-State Dynamics of Benzo[ghi]peryleneimide and Coroneneimide Derivatives2014

    • Author(s)
      T. Hasobe et al
    • Journal Title

      J. Phys. Chem. C

      Volume: 118 Issue: 14 Pages: 7710-7720

    • DOI

      10.1021/jp500761f

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Coordination Oligomers and Polymers of an Oxazole-appended Zinc Chlorophyll Derivative2014

    • Author(s)
      Yoshinao Shinozaki, Isao Yoshikawa, Koji Araki, Kazuaki Ohara, Kentaro Yamaguchi, Shin-ichiro Kawano, Kentaro Tanaka, Yasuyuki Araki, Takehiko Wada, Joe Otsuki
    • Journal Title

      Chemistry Letters

      Volume: 43 Issue: 6 Pages: 862-864

    • DOI

      10.1246/cl.140135

    • NAID

      130004868194

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Catalytic Bio-Supramolecular Photochirogenesis: Batch-Operated Enantiodifferentiating Photocyclodimerization of 2-Anthracenecarboxylate with Human Serum Albumin2013

    • Author(s)
      M. Nishijima, H. Kato, C. Yang, G. Fukuhara, T. Mori, Y. Araki, T. Wada, Y. Inoue
    • Journal Title

      ChemCatChem

      Volume: 5 Issue: 11 Pages: 3237-3240

    • DOI

      10.1002/cctc.201300160

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Time-resolved circular dichroism study on the photodynamics of cyclodextrin encapsulated aromatic molecules.2015

    • Author(s)
      M. Kuronuma, Y. Araki, S. SAKAMOTO, T. Wada
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      ホノルル.アメリカ合衆国
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 時間分解円二色性(CD)測定を活用したシクロデキス トリン包接錯体の光励起状態における動的挙動解析2015

    • Author(s)
      黒沼 慎, 荒木 保幸, 坂本 清志, 和田 健彦
    • Organizer
      第64回高分子討論会
    • Place of Presentation
      東北大学片平キャンパス(仙台市)
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] 生体機能分子の構造変化の高感度・高時間分解解 析を目指したCD測定装置の開発― 二重らせんDNA‐ポルフ ィリン誘導体相互作用の動的挙動解析の検討 ―2015

    • Author(s)
      佐藤 岳 仁 , 黒沼 慎 , 村上 慎 , 荒木 保幸 , 坂本 清志 , 和田 健 彦
    • Organizer
      第64回高分子討論会
    • Place of Presentation
      東北大学片平キャンパス(仙台市)
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] かさ高い置換基を導入したカチオン性ポルフィリンと二重らせんDNA間相互作用の時間分解CD法による検討2015

    • Author(s)
      荒木保幸,佐藤岳仁,黒沼慎,坂本清志, 和田健彦
    • Organizer
      2015年光化学討論会
    • Place of Presentation
      大阪市立大杉本キャンパス(大阪市)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] キラルなダイマー分子の光励起状態の時間分解円二色性測定による検討2015

    • Author(s)
      荒木 保幸・黒沼 慎・坂本 清志・森 直・井上 佳久・和田 健彦
    • Organizer
      日本化学会第95春季年会
    • Place of Presentation
      千葉県船橋市
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 時間分解 CD 測定によるシクロデキストリン包摂錯体の光励起ダイナミクスの検討2014

    • Author(s)
      荒木 保幸, 黒沼 慎, 坂本 清志, 和田 健彦
    • Organizer
      2014年光化学討論会
    • Place of Presentation
      札幌市
    • Year and Date
      2014-10-11 – 2014-10-13
    • Related Report
      2014 Research-status Report
  • [Presentation] 生体機能分子の構造変化の高感度・高時間分解能解析を目指したCD測定装置の開発(XX) -塩交換による四重鎖核酸構造変化の動的挙動解析-2013

    • Author(s)
      荒木 保幸, 濱田 芳生, 村上 慎, 坂本 清志, 和田 健彦
    • Organizer
      第23回バイオ・高分子シンポジウム
    • Place of Presentation
      東京都
    • Related Report
      2013 Research-status Report
  • [Presentation] キラルなペリレンジイミド 二量体の光励起挙動(II) -CDス ペクトルへのリンカー構造の影響-2013

    • Author(s)
      荒木 保幸,上 松 亮平,坂本 清志,和田 健彦
    • Organizer
      2013光化学討論会
    • Place of Presentation
      松山市
    • Related Report
      2013 Research-status Report
  • [Presentation] Cation Exchange Induced G-Quadruplex Structural Dynamics Studied by Circular Dichroism Spectroscopy2013

    • Author(s)
      Yasuyuki Araki , Yoshiki Hamada, Makoto Murakami, Seiji Sakamoto, Takehiko Wad
    • Organizer
      ISNAC2013 第40回国際核酸化学シンポジウム
    • Place of Presentation
      横浜市
    • Related Report
      2013 Research-status Report
  • [Presentation] Development of Circular Dichroism Detection System with Elliptically-polarized Light2013

    • Author(s)
      Yasuyuki Araki, Makoto Murakami, Seiji Sakamoto, Takehiko Wada
    • Organizer
      The 14th RIES-Hokudai International Symposium
    • Place of Presentation
      札幌市
    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi