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Novel Polymer Nanospheres for Serological Diagnosis of Malaria

Research Project

Project/Area Number 25410169
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bio-related chemistry
Research InstitutionGunma University

Principal Investigator

Oku Hiroyuki  群馬大学, 大学院理工学府, 准教授 (20301749)

Co-Investigator(Renkei-kenkyūsha) YANO Kazuhiko  国立研究開発法人国立国際医療研究センター, 研究所, 上級研究員 (30392393)
KANO Shigeyuki  国立研究開発法人国立国際医療研究センター, 研究所, 部長 (60233912)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords微粒子 / 高分子 / ペプチド / 抗原 / マラリア / 血清 / 抗体価 / 生体材料 / マラリア原虫 / 抗体価測定 / 高分子微粒子 / 感染履歴 / 放射線重合 / ナノ微粒子
Outline of Final Research Achievements

Malaria is a major public health problem especially in tropical and sub-tropical regions of the world. The methods most commonly used to diagnose malaria patients sera, such as IFAT and ELISA, are not easy methods to examine in the bedside and the clinical laboratory. Here, we wish to report novel diagnostic nanospheres for the detection of specific antibodies in malaria patients’ sera.
The peptide antigen was designed from a Plasmodium specific sequence in lactate dehydrogenase. The test-material is prepared by chemical coupling of the peptides to the surface of the nanosphere.
We have compared the reactivity against malaria patients’ sera collected in endemic regions of the Philippines: (a) Mixed infected-, (b) falciparum-, (c) vivax-malaria patients, and (d) feverish patients (malaria-negative). Successful results were observed for the nanosphere material, which showed good specificity for three kinds of malaria patients compared with feverish patients.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (19 results)

All 2015 2014 2013

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (12 results) (of which Invited: 1 results) Patent(Industrial Property Rights) (5 results) (of which Overseas: 3 results)

  • [Journal Article] Epitope Mapping of Monoclonal Antibodies Targeting the Loop Region of Plasmodium falciparum Enolase2015

    • Author(s)
      Hiroyuki Oku, Risa Onishi, Utako Arai, Yudai Kimoto, Keiichi Yamada; Kazuo Shinozuka; Yano, Kazuhiko; Kano, Shigeyuki
    • Journal Title

      Peptide Science 2014

      Volume: 2014 Pages: 275-278

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] マラリアの基礎とワクチン2014

    • Author(s)
      奥浩之、狩野繁之
    • Journal Title

      バムサジャーナル

      Volume: 26 Pages: 31-35

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 熱帯熱マラリア原虫酵素の部分ペプチドを用いた免疫血清のエピトープマッピング2015

    • Author(s)
      新井詩子、大西里咲、木本侑大、山田圭一、奥 浩之、狩野繁之、矢野和彦
    • Organizer
      日本化学会関東支部群馬地区地域懇談会
    • Place of Presentation
      群馬高専(群馬県前橋市箱田町)
    • Year and Date
      2015-12-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] マラリア原虫エノラーゼの部分ペプチドを用いたモノクローナル抗体作成とエピトープマッピング2015

    • Author(s)
      大西里咲、新井詩子、山田圭一、奥 浩之、狩野繁之、矢野和彦
    • Organizer
      日本化学会関東支部群馬地区地域懇談会
    • Place of Presentation
      群馬高専(群馬県前橋市箱田町)
    • Year and Date
      2015-12-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] 熱帯熱マラリア原虫酵素の部分ペプチドを用いた免疫血清のエピトープマッピング2015

    • Author(s)
      奥浩之、大西里咲、木本侑大、新井詩子、山田圭一、矢野和彦、狩野繁之
    • Organizer
      高分子学会関東支部第30回群馬・栃木地区講演会
    • Place of Presentation
      桐生市、群馬大学理工学部
    • Year and Date
      2015-03-06
    • Related Report
      2014 Research-status Report
  • [Presentation] Development Project of an All-Synthetic Nano/Micro-Sphere Vaccine Targeting Plasmodium falciparum Enolase2014

    • Author(s)
      Hiroyuki Oku, Risa Onishi, Utako Arai, Yudai Kimoto, Keiichi Yamada; Miho Ohue; Yoshiaki Ohyama; Tetsuya Nakamura; Yano, Kazuhiko; Kano, Shigeyuki
    • Organizer
      GUMI & AMDE 2014
    • Place of Presentation
      桐生市、桐生市民会館
    • Year and Date
      2014-12-05
    • Related Report
      2014 Research-status Report
  • [Presentation] Epitome Mapping of Inhibitory Antibodies Targeting the Loop Region of Plasmodium falciparum Enolase Using Synthetic Peptide Libraries2014

    • Author(s)
      Hiroyuki Oku, Risa Onishi, Utako Arai, Yudai Kimoto, Keiichi Yamada; Yano, Kazuhiko; Kano, Shigeyuki
    • Organizer
      JITMM 2014 & FBPZ8
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2014-12-02 – 2014-12-04
    • Related Report
      2014 Research-status Report
  • [Presentation] 熱帯熱マラリア原虫エノラーゼの部分ペプチドを用いたモノクローナル抗体作成とエピトープマッピング2014

    • Author(s)
      奥浩之、大西里咲、木本侑大、新井詩子、山田圭一、篠塚和夫、矢野和彦、狩野繁之
    • Organizer
      第51回ペプチド討論会
    • Place of Presentation
      徳島市、徳島大学蔵本キャンパス
    • Year and Date
      2014-10-22 – 2014-10-24
    • Related Report
      2014 Research-status Report
  • [Presentation] Epitope mapping of inhibitory monoclonal antibodies targeting the loop region of Plasmodium falciparum enolase using a combination of synthetic peptide libraries2014

    • Author(s)
      Oku, Hiroyuki; Arai, Utako; Onishi, Risa; Kimoto, Yudai; Moe Murakami; Shota Sato; Keiichi Yamada; Yano, Kazuhiko; Kano, Shigeyuki
    • Organizer
      ICOPA 2014
    • Place of Presentation
      Mexico City, Mexico
    • Year and Date
      2014-08-10 – 2014-08-15
    • Related Report
      2014 Research-status Report
  • [Presentation] 微粒子を用いたマラリアワクチンと抗体価検査キット2014

    • Author(s)
      奥浩之
    • Organizer
      首都圏北部4大学発 新技術説明会
    • Place of Presentation
      東京都千代田区、JST東京本部
    • Year and Date
      2014-06-20
    • Related Report
      2014 Research-status Report
  • [Presentation] LAMP法を用いた三日熱マラリア患者の鑑別診断:フィリピンでの実例2013

    • Author(s)
      矢野和彦、石上盛敏、奥浩之、Elena A. Villacorte、Pilarita T. Rivera、狩野繁之
    • Organizer
      第24回日本臨床寄生虫学会
    • Place of Presentation
      東大寺総合文化センター
    • Related Report
      2013 Research-status Report
  • [Presentation] マラリアの基礎とワクチン2013

    • Author(s)
      奥浩之
    • Organizer
      第24回トラベラーズワクチンフォーラム研修会
    • Place of Presentation
      国立国際医療研究センター
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Novel Polymer Nanospheres for Serological Diagnosis of Malaria2013

    • Author(s)
      H Oku, K Yano, S Kitamura, K Yamada, E A Villacorte, P T Rivera, S kano
    • Organizer
      JITMM 2013
    • Place of Presentation
      Centara Grand and Bangkok Convention Centre at Central World, Bangkok, Thailand
    • Related Report
      2013 Research-status Report
  • [Presentation] Gamma-Ray Polymerized Nanospheres for Serological Diagnosis of Malaria2013

    • Author(s)
      H Oku, K Yano, S Kitamura, K Yamada, E A Villacorte, P T Rivera, S kano
    • Organizer
      第62回米国熱帯医学会
    • Place of Presentation
      Marriott Wardman Park, Washington DC, USA
    • Related Report
      2013 Research-status Report
  • [Patent(Industrial Property Rights)] 熱帯熱マラリア原虫感染症の検査及び診断薬、並びに検査及び診断キット2015

    • Inventor(s)
      奥浩之、狩野繁之、矢野和彦
    • Industrial Property Rights Holder
      国立大学法人群馬大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2015-04-08
    • Related Report
      2015 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 熱帯熱マラリア原虫のエノラーゼ蛋白質の部分配列を用いた人工抗原とその製造方法2015

    • Inventor(s)
      奥浩之、狩野繁之、矢野和彦
    • Industrial Property Rights Holder
      国立大学法人群馬大学、独立行政法人国立国際医療研究センター
    • Industrial Property Rights Type
      特許
    • Filing Date
      2015-11-27
    • Related Report
      2015 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 熱帯熱マラリア原虫のエノラーゼ蛋白質の部分配列を用いた人工抗原とその製造方法2014

    • Inventor(s)
      奥浩之、矢野和彦、狩野繁之
    • Industrial Property Rights Holder
      奥浩之、矢野和彦、狩野繁之
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-080399
    • Filing Date
      2014-11-28
    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] 熱帯熱マラリア原虫粗抗原と血液試料中の抗体による反応を検出するための材料と方法、およびこれを用いたマラリア感染検査・抗体検査キット2014

    • Inventor(s)
      奥浩之、矢野和彦、狩野繁之
    • Industrial Property Rights Holder
      奥浩之、矢野和彦、狩野繁之
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-080399
    • Filing Date
      2014-04-09
    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] 三日熱マラリアと熱帯熱マラリアの双方を検出するペプチドおよび抗体検査材料2013

    • Inventor(s)
      奥浩之、北村慎也、山田圭一、狩野繁之、矢野和彦
    • Industrial Property Rights Holder
      奥浩之、北村慎也、山田圭一、狩野繁之、矢野和彦
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-11-15
    • Related Report
      2013 Research-status Report
    • Overseas

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Published: 2014-07-25   Modified: 2019-07-29  

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