Creation of novel poly-extremozymes by modifying protein surfaces
| Project/Area Number |
25410172
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Bio-related chemistry
|
|---|
| Research Institution | Tokyo Institute of Technology |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Keywords | 極限環境微生物 / 極限酵素 / 分子表面電荷 / タンパク質工学 / 進化分子工学 / 極限環境耐性 / 比活性 / キシラナーゼ / 耐アルカリ性 / 耐塩性 / キチナーゼ / 耐熱性 / 有機溶媒耐性 / アルカリキシラナーゼ |
|---|
| Outline of Final Research Achievements |
Alkalitolerancy, thermotolerancy and halotolerancy of a GH family 11 alkaline xylanase, XynJ, were improved by introducing basic amino acids on its protein surface. Halotolerancy and organic solvent-tolerancy of a GH family 18 halotolerant chitinase, ChiN1, were improved by introducing acidic amino acids on its protein surface. Alkalitolerancy and specific activity of a GH family 10 hyper-thermotolerant xylanase, XynTB, were improved by directed evolution.
|
Report
(4 results)
Research Products
(17 results)
-
-
-
-
[Journal Article] Identification of carotenoids from the extremely halophilic archaeon Haloarcula japonica2014
Author(s)
Rie Yatsunami, Ai Ando, Ying Yang, Shinichi Takaichi, Masahiro Kohno, Yuriko Matsumura, Hiroshi Ikeda, Toshiaki Fukui, Kaoru Nakasone, Nobuyuki Fujita, Mitsuo Sekine, Tomonori Takashina and Satoshi Nakamura
-
Journal Title
Frontiers Microbiology
Volume: 5
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] Gene Analysis, Expression, and Characterization of an Intracellular α-Amylase from the Extremely Halophilic Archaeon <i>Haloarcula japonica</i>2013
Author(s)
M. Onodera, R. Yatsunami, W. Tsukimura, T. Fukui, K. Nakasone, T. Takashina and S. Nakamura
-
Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 77
Issue: 2
Pages: 281-288
DOI
NAID
ISSN
0916-8451, 1347-6947
Related Report
Peer Reviewed
-
-
-
-
-
-
[Presentation] Characterization of a haloarchaealchitinase: Effect of aspartates, glutamates and lysines on its protein surface2013
Author(s)
Kinuka Toyama, Koichi Sakagami, Zhang Yang, Ann Ran, Motoaki Sato, KeitaOrishimo, YoshinoriHatori, Rie Yatsunami, TomonoriTakashina, Toshiaki Fukui, Satoshi Nakamura
Organizer
10th Asia-Pacific Chitin & Chitosan Symposium (第10回アジア・太平洋キチンキトサンシンポジュウム)・第27回キチン・キトサンシンポジウム
Place of Presentation
米子コンベンションセンター（鳥取県米子市）
Related Report
-
-
-
-
-
