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Construction of thin DNA-gel film with perfectly elasticity

Research Project

Project/Area Number 25410173
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bio-related chemistry
Research InstitutionYamagata University

Principal Investigator

Furusawa Hiroyuki  山形大学, 理工学研究科, 准教授 (60345395)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords水晶発振子 / アドミッタンス解析 / エネルギー散逸値 / 動的粘弾性測定 / 貯蔵弾性率 / 損失弾性率 / DNAネットワーク薄膜 / ポリジメチルシロキサン
Outline of Final Research Achievements

The aim of this study is (1) construction of a perfectly even structure using biomolecules of DNA strands, that allow us to provide a rigorous building block, and (2) measurement of dynamic viscoelasticity for the DNA-network gel made from the DNA strands. In this study, a tetrapod-like DNA structure was designed as a building block, and then the thin film of the DNA-network gel was prepared with the tetrapod-like DNA on a plate of Quartz-crystal microbalance, which can be used for dynamic viscoelasticity measurement. As a result, the DNA-network gel indicates the similar viscoelasticity as that of PDMS.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (23 results)

All 2016 2015 2014 2013 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Acknowledgement Compliant: 1 results) Presentation (16 results) (of which Int'l Joint Research: 2 results,  Invited: 4 results)

  • [Journal Article] DNA compaction determined by energy dissipation measurements on a quartz‑crystal oscillator2016

    • Author(s)
      H. Furusawa and T. Ozeki
    • Journal Title

      Microsyst. Technol.

      Volume: 22 Issue: 1 Pages: 65-71

    • DOI

      10.1007/s00542-015-2655-8

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Kinetic characterization of small DNA-binding molecules interacting with a DNA strand on a quartz crystal microbalance2016

    • Author(s)
      H. Furusawa, H. Nakayama, M. Funasaki, and Y. Okahata
    • Journal Title

      Anal. Biochem.

      Volume: 492 Pages: 34-42

    • DOI

      10.1016/j.ab.2015.09.015

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In situ monitoring of structural changes during formation of 30S translation initiation complex by energy dissipation measurement using 27-MHz quartz-crystal microbalance2014

    • Author(s)
      H. Furusawa, Y. Tsuyuki, S. Takahashi, and Y. Okahata
    • Journal Title

      Anal. Chem.

      Volume: 86 Issue: 11 Pages: 5406-5415

    • DOI

      10.1021/ac500487b

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Arginine Arrangement of Bacteriophage λ N-Peptide Plays a Role as a Core Motif in GNRA Tetraloop RNA Binding2014

    • Author(s)
      H. Furusawa, S. Fukusyo, and Y. Okahata
    • Journal Title

      ChemBioChem

      Volume: 15 Issue: 6 Pages: 865-871

    • DOI

      10.1002/cbic.201300809

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mechanism of Thiol-Disulfide Exchange Reactions between DsbA and DsbB over a Wide pH Range2013

    • Author(s)
      K. Yazawa, H. Furusawa, and Y. Okahata
    • Journal Title

      Chem. Lett.

      Volume: 42 Issue: 3 Pages: 241-243

    • DOI

      10.1246/cl.2013.241

    • NAID

      10031155766

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Translation Enhancer Improves the Ribosome Liberation from Translation Initiation2013

    • Author(s)
      S. Takahashi, H. Furusawa, T. Ueda, and Y. Okahata
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 135 Issue: 35 Pages: 13096-13106

    • DOI

      10.1021/ja405967h

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Real-time Monitoring of Intermediates Reveals the Reaction Pathway in the Thiol-Disulfide Exchange between Disulfide Bond Formation Protein A (DsbA) and B (DsbB) on a Membrane-immobilized Quartz Crystal Microbalance (QCM) System2013

    • Author(s)
      K. Yazawa, H. Furusawa, and Y. Okahata
    • Journal Title

      J. Biol. Chem.

      Volume: 288 Issue: 50 Pages: 35969-35981

    • DOI

      10.1074/jbc.m113.519876

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] DNAゲルネットワーク・フィルムの作製2016

    • Author(s)
      古澤 宏幸、薛 シン瑶
    • Organizer
      第26回バイオ高分子シンポジウム
    • Place of Presentation
      東京工業大学(東京)
    • Year and Date
      2016-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Observation of conformational changes of a functional RNA by using a quartz crystal microbalance with energy dissipation technique2015

    • Author(s)
      H. Furusawa
    • Organizer
      2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015)
    • Place of Presentation
      Hawaii Convention Center(Honolulu, Hawaii)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Hydration and viscoelasticity measurements of biocompatible polymers by using quartz-crystal microbalance with energy dissipation technique2015

    • Author(s)
      S. Xue, S. Kobayashi, M. Tanaka, H. Furusawa
    • Organizer
      2015 International Chemical Congress of Pacific Basin Societies (Pacifichem 2015)
    • Place of Presentation
      Hawaii Convention Center(Honolulu, Hawaii)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 水晶発振子マイクロバランス(QCM)法について2015

    • Author(s)
      古澤 宏幸
    • Organizer
      第9回QCM研究会
    • Place of Presentation
      機械振興会館(東京)
    • Year and Date
      2015-08-28
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 生体適合性を示す材料表面の物性評価2015

    • Author(s)
      薛 シン瑶、小林 慎吾、田中 賢、古澤 宏幸
    • Organizer
      第25回バイオ高分子シンポジウム
    • Place of Presentation
      東京工業大学(東京)
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] 水晶発振子エネルギー散逸測定法を用いた血液適合性高分子の物性評価2015

    • Author(s)
      薛 シン瑶、小林 慎吾、田中 賢、古澤 宏幸
    • Organizer
      日本化学会第95春季年会(2015)
    • Place of Presentation
      日本大学理工学部船橋キャンパス(千葉)
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] Evaluation of Physical Properties of Bio-related Polymers based on Energy Dissipation of a Quartz-Crystal Microbalance2014

    • Author(s)
      S. Xue, S. Kobayashi, M. Tanaka, H. Furusawa
    • Organizer
      The 10th SPSJ International Polymer Conference (IPC2014)
    • Place of Presentation
      つくば国際会議場(茨城県・つくば)
    • Year and Date
      2014-12-02 – 2014-12-05
    • Related Report
      2014 Research-status Report
  • [Presentation] Measurements of Hydration Amount and Viscoelasticity of Biocompatible Polymers using Quartz-Crystal Microbalance with Dissipation Technique2014

    • Author(s)
      S. Xue, S. Kobayashi, M. Tanaka, H. Furusawa
    • Organizer
      The 2nd International Conference of Smart System Engineering 2014 (SmaSys2014)
    • Place of Presentation
      伝国の杜(山形・米沢)
    • Year and Date
      2014-10-15 – 2014-10-17
    • Related Report
      2014 Research-status Report
  • [Presentation] DNAの柱で支える流動性脂質細胞膜の構築2014

    • Author(s)
      古澤 宏幸
    • Organizer
      第8回QCM研究会
    • Place of Presentation
      機械振興会館(東京)
    • Year and Date
      2014-08-22
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 全反射蛍光顕微鏡を用いたDNA二重鎖形成の一分子動力学2014

    • Author(s)
      古澤 宏幸、矢澤 健二郎、岡畑 惠雄
    • Organizer
      第24回バイオ高分子シンポジウム
    • Place of Presentation
      東京工業大学(東京)
    • Year and Date
      2014-07-24 – 2014-07-25
    • Related Report
      2014 Research-status Report
  • [Presentation] 全反射蛍光顕微鏡を用いたDNA支持型脂質膜上での膜タンパク質の動的挙動解析

    • Author(s)
      古澤 宏幸、辻 健太郎、岡畑 惠雄
    • Organizer
      第23回バイオ高分子シンポジウム
    • Place of Presentation
      東京工業大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 水晶発振子アドミッタンス法を用いるRNA鎖の動的構造変化の追跡

    • Author(s)
      古澤 宏幸、春原 有美子、吉田 亜矢、岡畑 惠雄
    • Organizer
      第62回高分子討論会
    • Place of Presentation
      金沢大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 水晶発振子アドミッタンス法を用いるHfqのRNAシャペロン活性の動的解析

    • Author(s)
      古澤 宏幸、吉田 亜矢、岡畑 惠雄
    • Organizer
      第7回バイオ関連化学シンポジウム
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 生体分子と高分子の粘弾性を測る

    • Author(s)
      古澤 宏幸
    • Organizer
      関東高分子若手研究会2013秋の講演会
    • Place of Presentation
      東京工業大学
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 水晶発振子で生体分子の構造変化をみる

    • Author(s)
      古澤 宏幸
    • Organizer
      平成25年度東北地区先端高分子セミナー
    • Place of Presentation
      仙台秋保温泉
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 全反射蛍光顕微鏡を用いたDNA二重鎖形成の一分子観察(1)

    • Author(s)
      古澤 宏幸、矢澤 健二郎、岡畑 惠雄
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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