Development of the production system of functional carotenoids rare in nature using chloroplast-genome modified lettuce
| Project/Area Number |
25410177
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Ishikawa Prefectural University |
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Principal Investigator |
MISAWA Norihiko 石川県立大学, 生物資源環境学部, 教授 (30393466)
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| Co-Investigator(Kenkyū-buntansha) |
新藤 一敏 日本女子大学, 家政学部, 教授 (80350180)
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| Co-Investigator(Renkei-kenkyūsha) |
MAOKA Takashi 一般財団法人生産開発科学研究所, 食物機能研究室, 室長 (10157159)
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| Research Collaborator |
TAKEMURA Miho
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | カロテノイド / レタス / 葉緑体形質転換 / カプサンチン / アスタキサンチン / フリチエラキサンチン / ノストキサンチン / イドキサンチン / イソプレノイド / 代謝工学 |
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| Outline of Final Research Achievements |
Carotenoids are one of the representative natural pigments, and prevent human body from oxidative stress. As promising functional carotenoids rare in nature, we noted 4-Ketocapsanthin, 2,2’-Dihydroxyastaxanthin, and Nostoxanthin. Plasmids for the biosynthesis of these pigments in the chloroplasts were constructed, and used to obtain respective chloroplast-genome modified (CGM) lettuce plants. Pigment analysis was carried out on these CGM lettuce plants. CGM lettuce that synthesized ketocarotenoids such as Astaxanthin and Fritschiellxanthin (4-Ketolutein; promising functional carotenoids rare in nature), which had already constructed in the beginning of the project, were cultivated with large scale, and supplied for experiments on plant physiology and confirmation study as food safety.
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Report
(5 results)
Research Products
(17 results)
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[Journal Article] The formation and sequestration of non-endogenous ketocarotenoids in transgenic Nicotiana glauca2017
Author(s)
Mortimer, C.L., Misawa, N., Perez-Fons, L., Robertson, F.P., Harada, H., Bramley, P.M., Fraser, P.D.
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Journal Title
Plant Physiology
Volume: -
Issue: 3
Pages: 1617-1635
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Novel carotenoids produced on the interaction of the foreign carotenoid ketolase CrtW and endogenous epoxycarotenoids unique to sweetpotato tubers2016
Author(s)
Maoka, T., Otani, M., Khan, M. Zubair, Takemura, M., Hattan, J., Misawa, N.
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Journal Title
Tetrahedron Letter
Volume: 57
Issue: 42
Pages: 4746-4748
DOI
Related Report
Peer Reviewed
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[Journal Article] Construction of transplastomic lettuce (Lactuca sativa) dominantly producing astaxanthin fatty acid esters and detailed chemical analysis of generated carotenoids2014
Author(s)
Harada, H., Maoka, T., Osawa, A., Hattan, J., Kanamoto, H., Shindo, K., Otomatsu, T., and Misawa, N.
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Journal Title
Transgenic Research
Volume: 23
Pages: 303-315
Related Report
Peer Reviewed
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[Presentation] Pathway engineering of sweetpotato plants for biosynthesis of astaxanthin and large varieties of ketocarotenoids2017
Author(s)
Misawa, N., Maoka, T., Hattan, J., Takemura, M., Choi, SK., Shindo, K., Otani, M.
Organizer
The 18th International Symposium on Carotenoids
Place of Presentation
Lucerne、スイス
Year and Date
2017-07-09
Related Report
Int'l Joint Research
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