Development of pH-responsive fluorescent nucleosides for SNPs detection
| Project/Area Number |
25410180
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Nihon University |
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Principal Investigator |
SAITO Yoshio 日本大学, 工学部, 准教授 (40385985)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 蛍光プローブ / pH環境 |
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| Outline of Final Research Achievements |
We have synthesized various pH-responsive fluorescent deoxyuridine derivatives containing pyridine skeleton. These fluorescent nucleosides exhibited distinctive fluorescence at 470-550 nm in aqueous solvents at basic to neutral pH values. Interestingly, 2-aminopyridine derivative exhibited strong fluorescence emission at 470 nm at pH range of 1.6-3.0 with a pKa value of 3.4 whereas exhibited emission at 550 nm at pH > 4.0. Such pH-sensitive fluorescent nucleosides can be used as fluorescence switch for monitoring pH change in biological systems.
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Report
(4 results)
Research Products
(6 results)
