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Development of intracellular delivery method of proteins by hetero-dimerized leucine zippers

Research Project

Project/Area Number 25410181
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bio-related chemistry
Research InstitutionKinki University

Principal Investigator

KITAMATSU Mizuki  近畿大学, 理工学部, 講師 (60379716)

Co-Investigator(Kenkyū-buntansha) MICHIUE Hiroyuki  岡山大学, 大学院医歯薬学総合研究科, 助教 (20572499)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsペプチド / DDS / 細胞 / ロイシンジッパー / 細胞内運搬ペプチド / p53タンパク質 / Nanogタンパク質 / ペプチド核酸 / p53 / Nanog / Beclin-1
Outline of Final Research Achievements

For purpose of treatment of disease, we develop a method for delivering a functional proteins or functional peptides into cells. In this work, we synthesized an autophagy-inducing peptide (Beclin 1) modified with a hetero-dimerization leucine zipper peptide (LzK). We also synthesized a cell-penetrating peptide modified with a pair of LzK (LzE). When these peptides were mixed, Beclin 1 was successfully delivered into the cell, and the peptide induced autophagy.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (5 results)

All 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Novel leucine zipper motif-based hybrid peptide delivers functional peptide cargo inside cells.2015

    • Author(s)
      Hakata, Y., S. Tsuchiya, H. Michiue, T. Ohtsuki, H. Matsui, M. Miyazawa, and M. Kitamatsu
    • Journal Title

      Chem. Comm.

      Volume: 51 Issue: 2 Pages: 413-416

    • DOI

      10.1039/c4cc07459a

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Peptide nucleic acid that conjugates with a cell-penetrating peptide via a disulfide bond for detecting a specific small RNA inside cells2015

    • Author(s)
      Mizuki Kitamatsu and Takashi Ohtsuki
    • Organizer
      Pacifichem2015
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ペプチドハイブリッド法によるオートファジー誘導ペプチドの細胞内輸送2014

    • Author(s)
      鷺森直人、北松瑞生、博多義之、宮澤正顯
    • Organizer
      第29回関西地区ペプチドセミナー
    • Place of Presentation
      大阪府・吹田市・関西大学(千里山キャンパス)
    • Year and Date
      2014-11-29
    • Related Report
      2014 Research-status Report
  • [Presentation] ペプチド‐ペプチド ハイブリッド法によるオートファジー誘導ペプチドの効果的輸送2014

    • Author(s)
      鷺森直人、博多義之、北松瑞生
    • Organizer
      第46回若手ペプチド夏の勉強会
    • Place of Presentation
      京都府・宮津市・京都府立青少年海洋センター
    • Year and Date
      2014-08-05
    • Related Report
      2014 Research-status Report
  • [Presentation] Delivery of peptides into cells by peptide-peptide hybrid method2014

    • Author(s)
      M. Kitamatsu, T. Ohtsuki
    • Organizer
      15th Tetrahedron Symposium
    • Place of Presentation
      London, UK
    • Year and Date
      2014-06-26
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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