Exploration of optimized chemical structure of siRNA based on the functional structure of human argonaute 2 protein
| Project/Area Number |
25410182
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Kinki University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 核酸化学 / 遺伝子サイレンシング / 化学修飾siRNA / アルゴノート2 / 核酸医薬 / オフターゲット効果 / 核酸関連化学 / アルゴノート２ / 遺伝子デリバリー |
|---|
| Outline of Final Research Achievements |
In the present study, we could demonstrate that chemical modification of siRNA increased the probability of the selection of antisense strand by Ago2, that conjugation of siRNA with signal peptides made it possible to control the intracellular localization of siRNA, and that non-covalent complexation of siRNA with the designed amphiphilic peptide improved cellular uptake and biological stability of siRNA. By a combination of further chemical modification, conjugation and complexation with a variety of functional molecules, we believe we can design an optimized structure of siRNA therapeutics which can effectively penetrate into cells and bind to the target molecule with a good affinity and specificity, without any side effects.
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Report
(4 results)
Research Products
(34 results)
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[Presentation] GENE SILENCING BY CATIONIC SIRNA2014
Author(s)
Ayumi Takashina, Hikari Kayano, Tomohiro Emi, Madoka Naemura, Yojiro Kotake, Jumpei Ariyoshi, Asako Yamayoshi, Akira Murakami and Masayuki Fujii
Organizer
The 41st International Symposium on Nucleic Acids Chemistry
Place of Presentation
北九州国際会議場(福岡県北九州市）
Year and Date
2014-11-05 – 2014-11-07
Related Report
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[Presentation] CATIONIC CHARGE ON SIRNA AND RNAI EFFECT2014
Author(s)
Ayumi Takashina, Hikari Kayano, Tomohiro Emi, Kana Murakami, Noriko Ogawa, Jumpei Ariyoshi, Asako Yamayoshi and Masayuki Fujii
Organizer
XXI Round Table on Chemical Biology of Nucleic Acids
Place of Presentation
ポズナン大学（ポーランド、ポズナン）
Year and Date
2014-08-24 – 2014-08-29
Related Report
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