| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In the present study, we could demonstrate that chemical modification of siRNA increased the probability of the selection of antisense strand by Ago2, that conjugation of siRNA with signal peptides made it possible to control the intracellular localization of siRNA, and that non-covalent complexation of siRNA with the designed amphiphilic peptide improved cellular uptake and biological stability of siRNA. By a combination of further chemical modification, conjugation and complexation with a variety of functional molecules, we believe we can design an optimized structure of siRNA therapeutics which can effectively penetrate into cells and bind to the target molecule with a good affinity and specificity, without any side effects.
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