| Project/Area Number |
25410189
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Green/Environmental chemistry
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Matsuda Tomoko 東京工業大学, 生命理工学研究科, 准教授 (10319494)
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| Co-Investigator(Kenkyū-buntansha) |
YAMANAKA Rio 姫路獨協大学, 薬学部, 講師 (40454764)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | グリーンケミストリー / 酵素 / 酸素 / 二酸化炭素 |
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| Outline of Final Research Achievements |
The oxidation and reduction reactions with risk-free reaction conditions were developed. The overexpressed enzymes (oxidase from Fusarium and reductase from Geotrichum candidum) using E-coli were used for the reactions, resulting in the very high efficiencies. Both enzymes have very wide substrate specificities, and excellent enantioselectivities. Especially, biotransformations of the challenging substrates for asymmetric synthesis were found to be also successful. Moreover, the structure of the G. candidum reductase was investigated by modeling and X-ray crystal structure determination to examine the mechanism to succeed in the excellent enantioselectivity.
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