Trypsin-immobilized nanosheets for increased proteolytic stability
| Project/Area Number |
25410230
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Polymer/Textile materials
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| Research Institution | Kumamoto University |
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Principal Investigator |
Sakata Masayo 熊本大学, 自然科学研究科, 准教授 (60187391)
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| Co-Investigator(Renkei-kenkyūsha) |
TANIGUCHI TAKAAKI 熊本大学, 大学院自然科学研究科, 助教 (50583415)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 酸化グラフェン / ナノシート / トリプシン / 固定化酵素 / 酵素活性 / 自己消化 / 酵素安定性 / 酸化グラフェンナノシート / プロテアーゼ / ナノ材料 / 酵素担体 / 磁性粒子 |
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| Outline of Final Research Achievements |
In this paper, we report a simple and natural method for the preparation of enzyme-immobilized grapheme oxide nanosheets (GO sheets) by the diimide-activated amidation of GO-bound carboxylic acids. Trypsin was used as a model enzyme for the investigation. In order to study the actual stabilization of immobilized trypsin, we also compared the thermal and chemical stabilities of the immobilized trypsin with the free-trypsin stabilities under various conditions. The trypsin-immobilized GO sheets (1.9 mg per mg-GO sheets) showed significantly higher thermal and chemical stabilities than the free form. After heat treatment at 50 °C for 6 h, the trypsin-GO sheets retained 80% of its initial activity, but free trypsin only retained 4% of its initial activity.
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Report
(4 results)
Research Products
(21 results)
