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Investigation of a Dynamic Mechanosensing System by Measuring Natural Modes of Vibration of a Cell

Research Project

Project/Area Number 25420179
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Dynamics/Control
Research InstitutionYokohama National University

Principal Investigator

SHIRAISHI TOSHIHIKO  横浜国立大学, 環境情報研究科(研究院), 准教授 (30361877)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords機械力学・制御 / 細胞 / 骨 / 振動学 / 振動モード / 固有振動数 / モデル化 / 力学センサ
Outline of Final Research Achievements

This report describes the micron-sized measurement of natural modes of vibration of a cell under mechanical vibration and the relationship between local deformation and biochemical reaction in a cell. As a result, we developed the experimental system for the measurement of natural modes of vibration for a living cell and confirmed its feasibility. Furthermore, we measured the relationship between the displacement of actin cytoskeletons and the maximum value of the change rate of calcium ion concentration of a cell. This indicates that the mechanical sensing system of a cell is partially clarified.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (30 results)

All 2016 2015 2014 2013 Other

All Presentation (28 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Remarks (2 results)

  • [Presentation] 細胞移動に対する焦点接着斑での繰り返しひずみの振幅と振動数の影響2016

    • Author(s)
      福野智大
    • Organizer
      日本機械学会第28回バイオエンジニアリング講演会
    • Place of Presentation
      東京
    • Year and Date
      2016-01-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 細胞骨格の変形分布とカルシウム応答の同時計測による細胞の力覚システムの検討2016

    • Author(s)
      堀口敦史
    • Organizer
      日本機械学会第28回バイオエンジニアリング講演会
    • Place of Presentation
      東京
    • Year and Date
      2016-01-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 細胞の移動と牽引力に対する焦点接着斑での繰り返しひずみの影響2015

    • Author(s)
      福野智大
    • Organizer
      第22回奈良・横浜・京都バイオメカニクスカンファレンス
    • Place of Presentation
      橿原
    • Year and Date
      2015-12-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] 細胞骨格の変形分布を考慮した培養骨芽細胞の力覚システムの検討2015

    • Author(s)
      堀口敦史
    • Organizer
      第22回奈良・横浜・京都バイオメカニクスカンファレンス
    • Place of Presentation
      橿原
    • Year and Date
      2015-12-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] Effects of Cyclic Strain at Focal Adhesions on Migration of and Osteoblast2015

    • Author(s)
      Tomohiro Fukuno
    • Organizer
      2015 ASME International Mechanical Engineering Congress and Exposition
    • Place of Presentation
      ヒューストン(米国)
    • Year and Date
      2015-11-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Study on a Cell Mechanosensing System by Measuring Structural Deformation and Biochemical Response2015

    • Author(s)
      Atsushi Horiguchi
    • Organizer
      2015 ASME International Mechanical Engineering Congress and Exposition
    • Place of Presentation
      ヒューストン(米国)
    • Year and Date
      2015-11-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 骨芽細胞の移動に対する焦点接着部での繰り返しひずみの振動数の影響2015

    • Author(s)
      福野智大
    • Organizer
      日本機械学会機械力学・計測制御部門講演会
    • Place of Presentation
      弘前
    • Year and Date
      2015-08-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] 構造変形と生化学応答の同時計測による細胞の力学刺激感受システムの検討2015

    • Author(s)
      堀口敦史
    • Organizer
      日本機械学会機械力学・計測制御部門講演会
    • Place of Presentation
      弘前
    • Year and Date
      2015-08-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] 細胞移動に対する焦点接着斑での繰り返しひずみの振動数の影響2015

    • Author(s)
      福野 智大
    • Organizer
      日本機械学会関東支部第21期総会・講演会
    • Place of Presentation
      横浜
    • Year and Date
      2015-03-20 – 2015-03-21
    • Related Report
      2014 Research-status Report
  • [Presentation] 構造変形と生化学応答の同時計測による細胞の力覚システムの検討2015

    • Author(s)
      堀口 敦史
    • Organizer
      日本機械学会関東支部第21期総会・講演会
    • Place of Presentation
      横浜
    • Year and Date
      2015-03-20 – 2015-03-21
    • Related Report
      2014 Research-status Report
  • [Presentation] 細胞移動に対する焦点接着斑での繰り返しひずみの影響2015

    • Author(s)
      福野 智大
    • Organizer
      日本機械学会第27回バイオエンジニアリング講演会
    • Place of Presentation
      新潟
    • Year and Date
      2015-01-09 – 2015-01-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 力学刺激に対する細胞の構造変形と生化学応答との関係2015

    • Author(s)
      堀口 敦史
    • Organizer
      日本機械学会第27回バイオエンジニアリング講演会
    • Place of Presentation
      新潟
    • Year and Date
      2015-01-09 – 2015-01-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 機械的振動下における細胞間接着の変化を考慮した細胞増殖メカニズムの検討2015

    • Author(s)
      石井 明紀
    • Organizer
      日本機械学会第27回バイオエンジニアリング講演会
    • Place of Presentation
      新潟
    • Year and Date
      2015-01-09 – 2015-01-10
    • Related Report
      2014 Research-status Report
  • [Presentation] Effects of Mechanical Vibration Stimulation on Cultured Osteoblasts2014

    • Author(s)
      Akinori Ishii
    • Organizer
      24th Annual Meeting of MRS-Japan 2014
    • Place of Presentation
      横浜
    • Year and Date
      2014-12-10 – 2014-12-12
    • Related Report
      2014 Research-status Report
  • [Presentation] Effects of Mechanical Vibration on Mutilayering of Cultured Osteoblasts2014

    • Author(s)
      Toshihiko Shiraishi
    • Organizer
      ASME 2014 International Mechanical Engineering Congress & Exposition
    • Place of Presentation
      モントリオール(カナダ)
    • Year and Date
      2014-11-14 – 2014-11-20
    • Related Report
      2014 Research-status Report
  • [Presentation] 細胞に対する焦点接着部での繰り返しひずみの影響2014

    • Author(s)
      福野 智大
    • Organizer
      日本機械学会機械力学・計測制御部門講演会(D&D2014)
    • Place of Presentation
      東京
    • Year and Date
      2014-08-26 – 2014-08-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 内部構造の変形を考慮した細胞の力学刺激感受システムの検討2014

    • Author(s)
      堀口 敦史
    • Organizer
      日本機械学会機械力学・計測制御部門講演会(D&D2014)
    • Place of Presentation
      東京
    • Year and Date
      2014-08-26 – 2014-08-29
    • Related Report
      2014 Research-status Report
  • [Presentation] 機械的振動による細胞増殖促進メカニズムの検討2014

    • Author(s)
      石井 明紀
    • Organizer
      日本機械学会機械力学・計測制御部門講演会(D&D2014)
    • Place of Presentation
      東京
    • Year and Date
      2014-08-26 – 2014-08-29
    • Related Report
      2014 Research-status Report
  • [Presentation] Development of Magnetic Micropillers to Apply Cyclic Strain to Focal Adhesions of Cells2014

    • Author(s)
      Tomohiro Fukuno
    • Organizer
      13th Joint Symposium among Sister Universities in Mechanical Engineering
    • Place of Presentation
      横浜
    • Year and Date
      2014-08-15 – 2014-08-17
    • Related Report
      2014 Research-status Report
  • [Presentation] Study on a Cell Mechanosensing System Considering Deformation of Cytoskeletal Structure2014

    • Author(s)
      Atsushi Horiguchi
    • Organizer
      13th Joint Symposium among Sister Universities in Mechanical Engineering
    • Place of Presentation
      横浜
    • Year and Date
      2014-08-15 – 2014-08-17
    • Related Report
      2014 Research-status Report
  • [Presentation] Effects of Mechanical Vibration on Cell Proliferation2014

    • Author(s)
      Akinori Ishii
    • Organizer
      13th Joint Symposium among Sister Universities in Mechanical Engineering
    • Place of Presentation
      横浜
    • Year and Date
      2014-08-15 – 2014-08-17
    • Related Report
      2014 Research-status Report
  • [Presentation] 培養骨芽細胞の増殖と細胞間接着に対する機械的振動の影響2014

    • Author(s)
      石井明紀
    • Organizer
      日本機械学会第26回バイオエンジニアリング講演会
    • Place of Presentation
      仙台
    • Related Report
      2013 Research-status Report
  • [Presentation] 大きなひずみ勾配をもつ繰り返し伸展刺激に対する培養骨芽細胞の応答2014

    • Author(s)
      小倉勇己
    • Organizer
      日本機械学会第26回バイオエンジニアリング講演会
    • Place of Presentation
      仙台
    • Related Report
      2013 Research-status Report
  • [Presentation] A Method for Applying High Cyclic Strain to Focal Adhesions and Measuring the Cell Response2013

    • Author(s)
      Toshihiko Shiraishi
    • Organizer
      The 2013 ASME International Mechanical Engineering Congress and Exposition
    • Place of Presentation
      サンディエゴ(米国)
    • Related Report
      2013 Research-status Report
  • [Presentation] Effects of Mechanical Vibration on Cell Proliferation and Differentiation2013

    • Author(s)
      Toshihiko Shiraishi
    • Organizer
      The 10th International Conference on Flow Dynamics
    • Place of Presentation
      仙台
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 勾配をもつ繰り返し伸展ひずみ場における骨芽細胞の適応的応答2013

    • Author(s)
      小倉勇己
    • Organizer
      日本機械学会機械力学・計測制御部門講演会(D&D2013)
    • Place of Presentation
      福岡
    • Related Report
      2013 Research-status Report
  • [Presentation] 機械的振動下における細胞増殖促進と細胞間接着の関係2013

    • Author(s)
      石井明紀
    • Organizer
      日本機械学会機械力学・計測制御部門講演会(D&D2013)
    • Place of Presentation
      福岡
    • Related Report
      2013 Research-status Report
  • [Presentation] 機械力学的視点による細胞研究2013

    • Author(s)
      白石俊彦
    • Organizer
      日本機械学会2013年度年次大会
    • Place of Presentation
      岡山
    • Related Report
      2013 Research-status Report
  • [Remarks] 横浜国立大学研究者総覧

    • URL

      http://er-web.jmk.ynu.ac.jp/html/SHIRAISHI_Toshihiko/ja.html

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
  • [Remarks] 横浜国立大学研究者総覧

    • URL

      http://er-web.jmk.ynu.ac.jp/html/SHIRAISHI_Toshihiko/ja.html

    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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