| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
We have assembled the asymmetric burst pulse system that can generate a single rectangular high voltage pulse in nanosecond and some low voltage burst pulses with both polarities from microsecond to millisecond. It was able to introduce some chemical substances into fertilized medaka eggs easily and efficiently with this pulse system. As a next challenge, a preparation of a transgenic organism with the pulse system was studied. Green Fluorescent Protein (GFP) plasmid vector was tried to introduce into fertilized medaka eggs by this system. GFP expression was observed in embryo at some pulse parameters, indicating that plasmid vector was transferred to medaka embryo cell and expressed coding GFP successfully. It became clear that the pulse shapes of both the pre- and the post-burst pulses and the length of H.V. short pulse were important parameters to induce the gene transfer. It is thought that this asymmetric burst pulse system would become a simple and easy incorporation system.
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