Study on Improvement of Gene Transfer Efficiency with Pulsed Power Technology
Project/Area Number |
25420276
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Power engineering/Power conversion/Electric machinery
|
Research Institution | Ariake National College of Technology |
Principal Investigator |
Kono Susumu 有明工業高等専門学校, その他部局等, 准教授 (30270375)
|
Research Collaborator |
TOMINAGA NOBUAKI
YAMAGUCHI AKEMI
Takehito Kaneko
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | パルスパワー / エレクトロポレーション / バーストパルス / 遺伝子 / GFP / 受精卵 / メダカ / 物質導入 / 遺伝子導入 / メダカ受精卵 / 線虫 / ラット / 極短パルス / バイオエレクトリクス / GFP |
Outline of Final Research Achievements |
We have assembled the asymmetric burst pulse system that can generate a single rectangular high voltage pulse in nanosecond and some low voltage burst pulses with both polarities from microsecond to millisecond. It was able to introduce some chemical substances into fertilized medaka eggs easily and efficiently with this pulse system. As a next challenge, a preparation of a transgenic organism with the pulse system was studied. Green Fluorescent Protein (GFP) plasmid vector was tried to introduce into fertilized medaka eggs by this system. GFP expression was observed in embryo at some pulse parameters, indicating that plasmid vector was transferred to medaka embryo cell and expressed coding GFP successfully. It became clear that the pulse shapes of both the pre- and the post-burst pulses and the length of H.V. short pulse were important parameters to induce the gene transfer. It is thought that this asymmetric burst pulse system would become a simple and easy incorporation system.
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Report
(4 results)
Research Products
(14 results)