| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
In the present work, we investigated the changes in permittivity and conductivity of the yeasts imposed by pulsed electric field with dielectrophoretic velocimetry. The relationship between interelectrode impedance due to integrated cells and the activities of membrane and growth was verified quantitatively. We also tried to establish a rapid and simple diagnostic system of cell dynamics.
As a major achievement, we quantified the damage degree of membrane using nucleic acid staining, and identified the difference of threshold against heating and applying of pulsed electric field. The electrical constants were deduced from the equivalent electrical circuit model in the comparison with dielectrophoretic velocity of yeast cells and interelectrode impedance. Further, the gene expression of damaged yeasts was detected and the judgment indicators of biological mutation were decided. In addition, the detection responses were reviewed under various pulse conditions.
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