| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Cryptosporidium oocysts are encased in a robust wall that is extremely resistant to environmental stressors such as chlorine used to disinfect potable water. Standard procedures used to extract DNA from oocysts such as the freeze-thaw method with DNA purification kit are time-consuming and expensive. Therefore, we developed the surfactant extraction treatment (SET) that efficiently extracts DNA from the oocyst. Furthermore, we evaluated the ability of SET to detect DNA directly from immunomagnetic bead-oocyst conjugates. As a result, the rate of DNA amplification using immunomagnetic separation-SET (IMS-SET) was greater than that using the standard procedures.
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