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adhesion behavior of microorganisms and biofilms on various materials revealed by AFM

Research Project

Project/Area Number 25420795
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Metal making/Resorce production engineering
Research InstitutionSuzuka National College of Technology

Principal Investigator

Nobumitsu Hirai  鈴鹿工業高等専門学校, その他部局等, 准教授 (50294020)

Co-Investigator(Kenkyū-buntansha) KANEMATSU HIDEYUKI  鈴鹿工業高等専門学校, 材料工学科, 教授 (10185952)
IKIGAI HAJIME  鈴鹿工業高等専門学校, 生物応用化学科, 教授 (60184389)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsバイオフィルム / 原子間力顕微鏡 / 微生物 / 細菌 / 細胞外重合物質 / 濡れ性 / 走査型プローブ顕微鏡 / 微生物腐食 / EPS
Outline of Final Research Achievements

The purpose of this study is to obtain knowledge about the materials on which the adhesion of microorganisms and biofilms is limited, revealed by AFM observation on the behavior of adhesion volume and shape of microorganisms and biofilms on various materials. It has been found that the adhesion volume and shape of microorganisms and biofilms are strongly dependent on the materials revealed by AFM observation. Adhesion work of the water containing some of the components of biofilms, such as alginate or rhamnolipid, on various materials has been also investigated, and the relationship between the adhesion work and the adhesion volume and shape of biofilms has been discussed.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (30 results)

All 2016 2015 2014 2013

All Journal Article (15 results) (of which Peer Reviewed: 12 results,  Acknowledgement Compliant: 4 results) Presentation (13 results) (of which Int'l Joint Research: 1 results) Book (2 results)

  • [Journal Article] Atomic force microscopy analysis of biofilms formed on different plastics2015

    • Author(s)
      N. Hirai, M. K. Mun, T. Masuda, H. Itoh, H. Kanematsu
    • Journal Title

      Materials Technology

      Volume: 30 Issue: sup5 Pages: B57-B60

    • DOI

      10.1179/1753555714y.0000000238

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 鉄鋼材料表面に形成された緑膿菌バイオフィルムの生体鉱物化2015

    • Author(s)
      生貝初, 平井信充, 黒田大介, 兼松秀行
    • Journal Title

      日本細菌学会誌

      Volume: 70 Pages: 143-143

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 鉄鋼材料に微生物腐食を引き起こす緑膿菌バイオフィルムの生体鉱物化2015

    • Author(s)
      生貝初,平井信充,小林正和,戸田裕之,黒田大介,兼松秀行
    • Journal Title

      CAMP-ISIJ

      Volume: 28 Pages: 469-470

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 各種基板上へのバイオフィルムの付着力評価の試み2015

    • Author(s)
      平井信充,金田貴文,黒木雅人,幸後健,兼松秀行
    • Journal Title

      CAMP-ISIJ

      Volume: 28 Pages: 471-472

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] FTIRおよびラマン分光法を用いた各種金属基板上に形成されたバイオフィルムの分析2015

    • Author(s)
      佐野勝彦,兼松秀行,平井信充,小川亜希子,幸後健,田中敏宏
    • Journal Title

      CAMP-ISIJ

      Volume: 28 Pages: 493-494

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] プラズマを用いた酸化物処理をした鉄鋼材料のバイオフィルム形成抑制効果2015

    • Author(s)
      加藤千聖,平井信充,佐野勝彦,兼松秀行,小川亜希子,幸後健
    • Journal Title

      CAMP-ISIJ

      Volume: 28 Pages: 497-498

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] EQCM法を用いたバイオフィルム形成解析の試み2015

    • Author(s)
      幸後健,駒田悠如,兼松秀行,平井信充,生貝初,佐野勝彦
    • Journal Title

      CAMP-ISIJ

      Volume: 28 Pages: 503-504

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 第4周期金属イオンのバイオフィルムによる選択的捕捉2015

    • Author(s)
      平井信充,岩田果久,杉田大地,兼松秀行
    • Journal Title

      CAMP-ISIJ

      Volume: 28 Pages: 507-508

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Biofilm formation on various plastics revealed by AFM2015

    • Author(s)
      Nobumitsu Hirai, Kar Mun Mah, Zhihong Bao, Hideyuki Kanematsu, Hajime Ikegai
    • Journal Title

      Proc. Asia Steel 2015

      Volume: 無 Pages: 76-77

    • Related Report
      2015 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] Anti-biofouling silane based composite coating and its structural-analysis by FIB-SEM2015

    • Author(s)
      Katsuhiko Sano, Hideyuki Kanematsu, Nobumitsu Hirai, Hajime Ikegai, Akiko Ogawa, Takeshi Kogo, Toshihiro Tanaka
    • Journal Title

      Proc. Asia Steel 2015

      Volume: 無 Pages: 154-155

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 各種合成樹脂上に形成されたバイオフィルムのAFMによる解析2014

    • Author(s)
      平井信充、マー カー ムン、増田智香、伊藤日出生、兼松秀行
    • Journal Title

      CAMP-ISIJ

      Volume: 27 Pages: 606-607

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] 卓上バイオフィルムリアクターの製作とバイオフィルム形成挙動2014

    • Author(s)
      西七恵,兼松秀行,三浦陽子,鬼頭みずき,平井信充,小川亜希子
    • Journal Title

      CAMP-ISIJ

      Volume: 27 Pages: 595-596

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] 生物付着抑制のためのシラン系樹脂コーティングとその評価法の検討2014

    • Author(s)
      佐野勝彦,兼松秀行,平井信充,小川亜希子,幸後健,田中敏宏
    • Journal Title

      CAMP-ISIJ

      Volume: 27 Pages: 597-598

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] LBRを用いた二つの複合材料のバイオファウリング挙動比較2014

    • Author(s)
      中西康夫,兼松秀行,三浦陽子,V. Arumugam,平井信充,小川亜希子
    • Journal Title

      CAMP-ISIJ

      Volume: 27 Pages: 602-603

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] 各種材料上に形成されるバイオフィルムの新しい評価分析解析手法2013

    • Author(s)
      兼松秀行,平井信充,三浦陽子,伊藤日出生,萩野唯,田中美穂
    • Journal Title

      CAMP-ISIJ

      Volume: 26 Pages: 664-665

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] EQCM法を用いたバイオフィルム形成挙動の解析2016

    • Author(s)
      幸後健, 駒田悠如, 平井信充,兼松秀行,生貝初,佐野勝彦
    • Organizer
      2016年電気化学会第83回大会
    • Place of Presentation
      大阪大学
    • Year and Date
      2016-03-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] 光学顕微鏡によるバイオフィルム観察のためのイオン液体を用いた試料固定の可能性2016

    • Author(s)
      兼松秀行,平井信充,綱島克彦,小川亜希子
    • Organizer
      2016年電気化学会第83回大会
    • Place of Presentation
      大阪大学
    • Year and Date
      2016-03-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] 固体表面上に形成したバイオフィルムによる水中クロムイオンの選択的濃縮2015

    • Author(s)
      平井信充,岩田果久, 杉田大地, 兼松秀行
    • Organizer
      第35回表面科学学術講演会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 常在菌バイオフィルムによる金属イオンの選択的濃縮2015

    • Author(s)
      岩田果久, 杉田大地, 平井信充,兼松秀行
    • Organizer
      第46回中部化学関係学協会支部連合秋季大会
    • Place of Presentation
      三重大学
    • Year and Date
      2015-11-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] Biofilm formation on various plastics revealed by AFM2015

    • Author(s)
      Nobumitsu Hirai, Kar Mun Mah, Zhihong Bao, Hideyuki Kanematsu, Hajime Ikegai
    • Organizer
      Asia Steel 2015
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2015-10-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 鈴鹿高専における創造工学教育の紹介~微生物電池を題材として~2014

    • Author(s)
      中川元斗,菱川湧輝,小林大士,飯田壮葵,丹羽菜月,〇平井信充
    • Organizer
      日本鉄鋼協会材料の組織と特性部会「微生物が促進する鉄鋼材料の腐食」第2回自主フォーラム
    • Place of Presentation
      秋田大学
    • Year and Date
      2014-12-09
    • Related Report
      2014 Research-status Report
  • [Presentation] グラッシーカーボン上に形成した緑膿菌バイオフィルムのAFM観察2014

    • Author(s)
      平井信充,小澤ひかり,生貝初
    • Organizer
      日本鉄鋼協会第168回秋季講演大会「微生物が促進する鉄鋼材料の腐食」自主フォーラム シンポジウム
    • Place of Presentation
      名古屋大学
    • Year and Date
      2014-09-25
    • Related Report
      2014 Research-status Report
  • [Presentation] A trial for MIC study using a circulation-type laboratory biofilm reactor.2014

    • Author(s)
      Hideyuki Kanematsu, Miwa Noda, Nobumitsu Hirai, Akiko Ogawa, Takeshi Kogo, Yoko Miura, Hideo Itoh
    • Organizer
      17th Inter. Cong. on Marine Corrosion and Fouling (ICMCF 2014)
    • Place of Presentation
      Singapore
    • Year and Date
      2014-07-09
    • Related Report
      2014 Research-status Report
  • [Presentation] Composite coating to control biofilm formation and MIC2014

    • Author(s)
      Hideyuki Kanematsu, Takeshi Kogo, Miwa Noda, Nobumitsu Hirai, Akiko Ogawa, Yoko Miura, Hideo Itoh, Michiko Yoshitake.
    • Organizer
      17th Inter. Cong. on Marine Corrosion and Fouling (ICMCF 2014)
    • Place of Presentation
      Singapore
    • Year and Date
      2014-07-09
    • Related Report
      2014 Research-status Report
  • [Presentation] Biofilm formation processes on sttel surface revealed by AFM2014

    • Author(s)
      Nobumitsu Hirai, Hideyuki Kanematsu, Takafumi Kanata, Hikari Ozawa, Hideo Itoh, Tomoka Masuda, Toshihiro Tanaka
    • Organizer
      17th Inter. Cong. on Marine Corrosion and Fouling (ICMCF 2014)
    • Place of Presentation
      Singapore
    • Year and Date
      2014-07-08
    • Related Report
      2014 Research-status Report
  • [Presentation] バイオフィルム形成過程の水中AFM観察に向けた基礎的検討2013

    • Author(s)
      平井信充,兼松秀行,生貝初
    • Organizer
      日本防菌防黴学会第40回年次大会
    • Place of Presentation
      千里ライフサイエンスセンター
    • Related Report
      2013 Research-status Report
  • [Presentation] 各種材料上に形成されるバイオフィルムの新しい評価分析解析手法2013

    • Author(s)
      兼松秀行,平井信充,三浦陽子,伊藤日出生,萩野唯,田中美穂
    • Organizer
      日本鉄鋼協会第166回秋季講演大会
    • Place of Presentation
      金沢大学 角間キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] Fundamental study for in-situ observation of biofilm in water by means of AFM2013

    • Author(s)
      Nobumitsu Hirai, Takafumi Kanata, Masanori Suzuki, Shigeru Katsuyama, Toshihiro Tanaka, Hideyuki Kanematsu
    • Organizer
      Materials Science and Technology (MS&T) 2013
    • Place of Presentation
      カナダ モントリオール
    • Related Report
      2013 Research-status Report
  • [Book] バイオフィルムとその工業利用2015

    • Author(s)
      兼松秀行・生貝初・黒田大介・平井信充
    • Total Pages
      156
    • Publisher
      米田出版
    • Related Report
      2014 Research-status Report
  • [Book] Biofilm and Materials Science "Energy Problems-Fuel Cell"2015

    • Author(s)
      Nobumitsu Hirai
    • Total Pages
      9
    • Publisher
      Springer
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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