| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Protein engineering study on a phospholipase D (PLD) was performed in to synthesize phosphatidylinositol (PI), a functional phospholipid, with high purity. Analysis of many PLD variants with mutations at its substrate-binding pocket successfully identified some variants with excellent positional specificity. In addition, trimming an unstable loop structure from PLD molecule lead to the creation of another variant with both improved stability and high positional specificity. Moreover, during the optimization of the reaction conditions, we found that very high concentration of NaCl enhanced the PI yield. Furthermore, we discovered another enzyme that hydrolyzes phosphatidic acid (a unwanted side product) from a microorganism by screening.
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