Development of engineered enzymes for synthesis of highly pure phosphatidylinositol
Project/Area Number |
25420830
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biofunction/Bioprocess
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Research Institution | Nagoya University |
Principal Investigator |
Iwasaki Yugo 名古屋大学, 生命農学研究科, 准教授 (50273214)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | phospholipase D / phosphatidylinositol / ホスホリパーゼD / ホスファチジルイノシトール / ホスホリパーゼ |
Outline of Final Research Achievements |
Protein engineering study on a phospholipase D (PLD) was performed in to synthesize phosphatidylinositol (PI), a functional phospholipid, with high purity. Analysis of many PLD variants with mutations at its substrate-binding pocket successfully identified some variants with excellent positional specificity. In addition, trimming an unstable loop structure from PLD molecule lead to the creation of another variant with both improved stability and high positional specificity. Moreover, during the optimization of the reaction conditions, we found that very high concentration of NaCl enhanced the PI yield. Furthermore, we discovered another enzyme that hydrolyzes phosphatidic acid (a unwanted side product) from a microorganism by screening.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Directing positional specificity in enzymatic synthesis of bioactive 1-phosphatidylinositol by protein engineering of a phospholipase D.2016
Author(s)
Damnjanovic, J., Kuroiwa, C., Tanaka, H., Ishida, K., Nakano, H., and Iwasaki, Y.
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Journal Title
Biotechnol. Bioeng.
Volume: 113
Issue: 1
Pages: 62-71
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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