Development of microbial catalyst for biomass refinery based on syngas platform
Project/Area Number |
25420832
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biofunction/Bioprocess
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Research Institution | Hiroshima University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 水素 / 二酸化炭素 / 合成ガス / エタノール / 発酵 / ホモ酢酸菌 / 代謝工学 / 酢酸 / バイオガス / 好熱性菌 / プロテオーム / 遺伝子工学 / 合成代謝工学 / バイオリファイナリー |
Outline of Final Research Achievements |
In this study, we aimed to evaluate the availability of useful metabolite production by means of synthetic biotechnology from syngas by Moorella thermoacetica as a host microorganism. After developing novel transformation system of Moorella with thermotolerant kanamycin resistant marker gene, we successfully constructed a mutant strain that mainly produced ethanol from fructose by the disruption of acetate synthetic pathway and introduction of alcohol synthetic pathway. Although the mutant lost fermentability of H2-CO2, it was recovered by the addition of sugar, suggesting that the mutant is a promising candidate for ethanol production by co-fermentation of carbohydrate and syngas.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Engineering of a functional thermostable kanamycin resistance marker for use in Moorella thermoacetica ATCC390732013
Author(s)
6. Yuki Iwasaki, Akihisa Kita, Shinsuke Sakai, Kazue, Takaoka, Shinichi Yano, Takahisa, Tajima, Junichi, Kato, Naomichi Nishio, Katsuji, Murakami, Yutaka Nakashimada
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Journal Title
FEMS Microbiology Letters
Volume: 343
Issue: 1
Pages: 8-12
DOI
Related Report
Peer Reviewed
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[Presentation] The Production of Ethanol with Metabolism Mutant of Thermophillic Bacteria Moorella thermoacetica ATCC390732015
Author(s)
Yuki Iwasaki, Koichiro Yoshida, Akihisa Kita, Shinsuke Sakai, Tomohiro Sho , Masahiro Saito, Katsuji Murakami, Takahisa Tajima, Junichi Kato, Yutaka Nakashimada
Organizer
The 4th Joint Conference on Renewable Energy and Nanotechnology
Place of Presentation
Matsuyama, Japan
Year and Date
2015-12-05
Related Report
Int'l Joint Research
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[Presentation] 合成ガス資化性好熱性細菌 Moorella thermoacetica 代謝変異株によるエタノール生産2015
Author(s)
吉田 嵩一郎, 岩崎 祐樹, 三好 佑季, 河合 優人, 喜多 晃久, 酒井 伸介, 庄 智裕, 斉藤 政宏, 村上 克治, 星野 保, 田島 誉久, 中島田 豊, 加藤 純一
Organizer
第67回 日本生物工学会 大会
Place of Presentation
鹿児島
Year and Date
2015-10-26
Related Report
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