| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In this study, we aimed to evaluate the availability of useful metabolite production by means of synthetic biotechnology from syngas by Moorella thermoacetica as a host microorganism. After developing novel transformation system of Moorella with thermotolerant kanamycin resistant marker gene, we successfully constructed a mutant strain that mainly produced ethanol from fructose by the disruption of acetate synthetic pathway and introduction of alcohol synthetic pathway. Although the mutant lost fermentability of H2-CO2, it was recovered by the addition of sugar, suggesting that the mutant is a promising candidate for ethanol production by co-fermentation of carbohydrate and syngas.
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